湖南中医药大学学报2018，Vol.38Issue(11)：1243-1247,5.DOI:10.3969/j.issn.1674-070X.2018.11.005
电针下合穴阳陵泉等对急性胆囊炎模型豚鼠血清TNF-α及胆囊组织nAchR α7 mRNA的影响
Effects of Electroacupuncture at Yanglingquan and Other Lower He-Sea Points on Serum TNF-α and Gallbladder nAChR α7 mRNA in Guinea Pig Model of Acute Cholecystitis
摘要
Abstract
Objective To compare the effects of electroacupuncture at Yanglingquan, Shangjuxu, Xiajuxu, and Zusanli points on serum tumor necrosis factor-α (TNF-α) and m RNA expression of α7 nicotinic acetylcholine receptor (nAChR α7) in the gallbladder in a guinea pig model of acute cholecystitis (AC), and to explore whether Yanglingquan, the lower He-sea point of the gallbladder, is relatively specific for treating the corresponding visceral disease. Methods Seventy-two healthy guinea pigs were randomly and equally divided into blank group, model group, Yanglingquan group, Shangjuxu group, Xiajuxu group, and Zusanli group, with equal numbers of male and female in each group. A model of AC was established by injection of Escherichia coli into the gallbladder of all guinea pigs except for the blank group. After model establishment, the four treatment groups received electroacupuncture for 5 days. The gallbladder was collected for HE staining and morphological analysis by microscopy.The serum level of TNF-α and expression of nAChR α7 mRNA in the gallbladder were measured by enzyme-linked immunosorbent assay and RT-PCR, respectively. Results Compared with the blank group, the other groups had varying degrees of pathological damages in the gallbladder according to HE staining and microscopic observation, as well as significantly lower expression of nAChR α7 mRNA and a significantly higher serum level of TNF-α (P＜0.01). Compared with the model group, thefour electroacupuncture groups had a significantly lower serum level of TNF-α and significantly higher expression of nAChRα7 mRNA in the gallbladder (P＜0.01). Compared with the Yanglingquan group, the Shangjuxu group, the Xiajuxu group, and the Zusanli group had a significantly higher serum level of TNF-α and significantly lower expression of nAChR α7 mRNA in the gallbladder (P＜0.01). Conclusion Electroacupuncture at the lower He-sea points of digestion-related organs has different degrees of therapeutic effects on the guinea pig model of AC, probably by regulating TNF-α and the expression of nAChR α7 m RNA and then inhibiting inflammatory factors and reducing inflammatory reaction. According to the comparative study, the Yanglingquan group has a better intervention effect than other treatment groups, suggesting that Yanglingquan point is specific for the gallbladder.关键词
急性胆囊炎/电针/下合穴/肿瘤坏死因子-α/乙酰胆碱受体α7信使核糖核酸/合治内府Key words
acute cholecystitis/electroacupuncture/lower He-sea point/tumor necrosis factor-α/α7 nicotinic acetylcholine receptor mRNA/treating visceral diseases with He-sea point分类
医药卫生引用本文复制引用
邓石峰,过灵香,凌希,易细芹,祁芳,张泓..电针下合穴阳陵泉等对急性胆囊炎模型豚鼠血清TNF-α及胆囊组织nAchR α7 mRNA的影响[J].湖南中医药大学学报,2018,38(11):1243-1247,5.基金项目
国家自然科学基金项目(81173327/H2718) （81173327/H2718）
