湖南中医药大学学报2018，Vol.38Issue(11)：1248-1252,5.DOI:10.3969/j.issn.1674-070X.2018.11.006
电针阳陵泉等下合穴对急性胆囊炎豚鼠组织高迁移率族蛋白B1的影响
Effect of Electroacupuncture at Yanglingquan and Other Lower He-Sea Points on the Expression of High-Mobility Group Box Protein B1 in Guinea Pig Model of Acute Cholecystitis
摘要
Abstract
Objective To observe the effect of electroacupuncture at Yanglingquan, Zusanli, Shangjuxu, and Xiajuxu points on the expression of high-mobility group box protein B1 (HMGB1), interleukin-1 beta (IL-1β), and nuclear factor kappa B messenger RNA (NF-κB mRNA) in the gallbladder tissue in a guinea pig model of acute cholecystitis (AC), and to explore the relative specificity of Yanglingquan, the lower He-sea point of the gallbladder, in the treatment of corresponding visceral disease.Methods Seventy-two healthy guinea pigs were randomly divided into six groups: normal group, model group, Yanglingquan group, Zusanli group, Shangjuxu group, and Xiajuxu group, with 6 males and 6 females in each group. All guinea pigs except those in the normal group were injected with Escherichia coli into the gallbladder to establish the AC model. After successful modeling and treatment for 5 days, gallbladder tissues were taken to measure the expression levels of HMGB1 and IL-1β by the immunohistochemical method, and the level of NF-κB mRNA was determined by RT-PCR. Results Compared with the model group, all the four lower He-sea groups had significantly lower transcription of NF-κB mRNA (P＜0.01). The levels of HMGB1 and IL-1βin the Yanglingquan group were significantly lower than those in the other groups (P＜0.01). Compared with the Yanglingquan group, the other lower He-sea groups had significantly higher levels of HMGB1, IL-1β, and NF-κB mRNA (P＜0.01 or P＜0.05). Conclusion Electroacupuncture at lower He-sea points in the stomach, intestine, and gallbladder may interfere with AC by inhibiting inflammatory factors, including HMGB1 and IL-β, in the gallbladder tissue of guinea pigs with AC, thus reducing activation of the NF-κB signaling pathway and alleviating the inflammation of the gallbladder. For the guinea pig model of AC, electroacupuncture at Yanglingquan has better efficacy than that at Zusanli, Shangjuxu, and Xiajuxu, indicating that electroacupuncture at Yanglingquan may have relative specificity in the treatment of AC.关键词
急性胆囊炎/电针/下合穴/豚鼠/高迁移率族蛋白B1/白介素-1β/核因子-κB信使核糖核酸Key words
acute cholecystitis/electroacupuncture/lower He-sea point/guinea pig/high-mobility group box protein B1/interleukin-1 beta/nuclear factor kappa B mRNA分类
医药卫生引用本文复制引用
祁芳,张泓,易细芹,邓石峰,周予婧,许明..电针阳陵泉等下合穴对急性胆囊炎豚鼠组织高迁移率族蛋白B1的影响[J].湖南中医药大学学报,2018,38(11):1248-1252,5.基金项目
国家自然科学基金面上项目(81173327) （81173327）
