湖南中医药大学学报2018，Vol.38Issue(11)：1267-1272,6.DOI:10.3969/j.issn.1674-070X.2018.11.010
穴位埋线疗法对缺血性认知障碍模型大鼠的学习记忆力及海马CA1区突触超微结构的影响
Effect of Acupoint Catgut Embedding Therapy on Learning, Memory, and Synaptic Ultrastructure in the Hippocampal CA1 Region in Rats with Ischemic Cognitive Impairment
摘要
Abstract
Objective To investigate the effect of acupoint catgut embedding therapy on learning, memory, and the morphology and ultrastructure of hippocampal CA1 region in rats with ischemic cognitive impairment, and to explore its therapeutic mechanism.Methods A total of 32 male specific pathogen-free Sprague-Dawley rats were randomly and equally divided into sham-operation group, model group, acupoint catgut embedding group, and medication group. A rat model of chronic ischemic cognitive impairment was established by permanent ligation of both common carotid arteries. The catgut was embedded in the Baihui, Dazhui, Shenshu, and Xuanzhong points once a week for 4 weeks. Rats in the medication group received intraperitoneal injection of0.33 mg/kg of monosialotetrahexosylganglioside sodium once a day for 4 weeks. Learning and memory abilities of rats were tested by Morris water maze. Nissl staining was used to observe the changes in pathology and area of the Nissl bodies inthe hippocampus. Transmission electron microscopy was used to observe ultrastructural changes in the hippocampal CA1 region.Results Compared with the sham-operation group, the model group had significantly reduced learning and memory abilities, significantly reduced area of the Nissl bodies in the hippocampus, and significantly different synaptic structure parameters (all P＜0.01);the synapse-related structures were damaged in the model group. Compared with the model group, the acupoint catgut embedding group had significantly improved learning and memory abilities, significantly larger area of the Nissl bodies, a significantly larger number of synapses in the hippocampal CAl region, a significantly elongated synaptic active area, a significantly narrowed synaptic gap, and a significantly thickened postsynaptic density (all P＜0.01). There were no significant differences in learning, memory, area of the Nissl bodies, or synaptic ultrastructure in the hippocampal CA1 region between the acupoint catgut embedding group and the medication group (P＞0.05). Conclusion Acupoint catgut embedding can reduce cognitive impairment in rats with ischemic cognitive impairment, probably by protection of the Nissl bodies in the hippocampus and the improvement of synaptic plasticity.关键词
缺血性认知障碍/穴位埋线/突触超微结构变化/海马CA1区/尼氏小体Key words
ischemic cognitive impairment/acupoint catgut embedding/ultrastructural changes in synapses/hippocampal CA1 region/Nissl body分类
医药卫生引用本文复制引用
黎帅,黄桂兰,张泓,邹莹洁,郭奎奎,邓多喜,赵东凤,谭洁..穴位埋线疗法对缺血性认知障碍模型大鼠的学习记忆力及海马CA1区突触超微结构的影响[J].湖南中医药大学学报,2018,38(11):1267-1272,6.基金项目
湖南省教育厅优秀青年项目(16B197) （16B197）
湖南省研究生创新项目(CX2017B453) （CX2017B453）
湖南省自然基金项目(2018JJ3384) （2018JJ3384）
