湖南中医药大学学报2018，Vol.38Issue(11)：1273-1277,5.DOI:10.3969/j.issn.1674-070X.2018.11.011
隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α蛋白及其mRNA表达的影响
Effect of Herbal Cake-Separated Moxibustion on the Expression of TNF-α Protein and Its mRNA in Lung Tissue of Chronic Bronchitis Model Rats
摘要
Abstract
Objective To observe the effect of herbal cake-separated moxibustion on the content of tumor necrosis factor-α (TNF-α) protein and its mRNA in the lung tissue of rats with chronic bronchitis (CB). Methods Forty-eight Sprague-Dawley rats were randomly and equally divided into four groups, including blank group, model group, herbal cake-separated moxibustion group, and paper pad-separated moxibustion group. CB model rats were prepared by modified fumigation. The general status of rats after herbal cake-separated moxibustion was observed and compared with that in the other three groups. The expression of TNF-α mRNA in rat lung was measured by RT-PCR, and the protein expression of TNF-α in lung tissues was measured by Western blot.Results The pathological injury of lung and trachea of rats in herbal cake-separated moxibustion group and paper pad-separated moxibustion group was improved compared with that in the model group. Herbal cake-separated moxibustion and paper padseparated moxibustion can reduce the expression of TNF-α mRNA and TNF-α protein in the lung tissues of CB rats (P＜0.05), and the effect of herbal cake-separated moxibustion was better (P＜0.05). Conclusion Herbal cake-separated moxibustion can improve the general symptoms and airway inflammation of CB rats, possibly by down-regulating the expression of TNF-α protein and its mRNA.关键词
慢性支气管炎/隔药饼灸/肿瘤坏死因子α/肿瘤坏死因子α信使RNAKey words
chronic bronchitis/herbal cake-separated moxibustion/tumor necrosis factor-α/tumor necrosis factor-α mRNA分类
医药卫生引用本文复制引用
孙璐,谭静,阳仁达,言芳,廖宗力,魏星,罗明..隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α蛋白及其mRNA表达的影响[J].湖南中医药大学学报,2018,38(11):1273-1277,5.基金项目
湖南省教育厅创新平台项目(16K068) （16K068）
湖南省大学生创新实验项目(017-132) （017-132）
