湖南中医药大学学报2018，Vol.38Issue(11)：1321-1325,5.DOI:10.3969/j.issn.1674-070X.2018.11.023
补阳还五汤对移植BMSCs在脊髓损伤局部存活、增殖及分化的影响
Effect of Buyang Huanwu Decoction on the Survival, Proliferation, and Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells After Spinal Cord Injury
摘要
Abstract
Objective To investigate the effect of Buyang Huanwu Decoction (BYHWD) on the survival, proliferation, and differentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) after spinal cord injury (SCI). Methods A total of60 male Sprague-Dawley rats were randomly divided into normal group, Qi deficiency-blood stasis group, BYHWD group, BMSCs group, and BYHWD+BMSCs group, with 12 rats in each group. All rats except those in the normal group were used to establish a model of SCI with Qi deficiency and blood stasis, and corresponding intervention was given for 28 consecutive days.The inclined plate test was used to observe the recovery of motor function of the forelimb, and immunohistochemistry was used to measure the local expression of 5-bromo-2'-deoxyuridine (BrdU), nestin, and glial fibrillary acidic protein (GFAP). Results On days 14 and 28 after surgery, the BYHWD+BMSCs group had significantly higher score of inclined plate test and rate of use of forelimb than the Qi deficiency-blood stasis group (P＜0.05), while the BYHWD group and the BMSCs group had significantlylower data than the BYHWD+BMSCs group (P＜0.05). Immunohistochemistry showed that there were no BrdU-positive cells in the normal group, the Qi deficiency-blood stasis group, and the BYHWD group; only a small number of BrdU-positive cells were observed in the BMSCs group, and a large number was observed in the BYHWD+BMSCs group. A small number of nestin-positive cells were observed in the normal group; there was a slight increase in the number of nestin-positive cells due to stress in the Qi deficiency-blood stasis group and a significant increase in the BYHWD group, the BMSCs group, and the BYHWD +BMSCs group, and the BYHWD+BMSCs group had a significantly higher number than the BYHWD group and the BMSCs group (P＜0.01). The BYHWD group, the BMSCs group, and the BYHWD+BMSCs group had significantly lower expression of GFAP than the Qi deficiency-blood stasis group (P＜0.01), while the BYHWD group and the BMSCs group had significantly higher expression of GFAP than the BYHWD+BMSCs group (P＜0.01). Conclusion BYHWD can enhance the survival and proliferation of transplanted BMSCs in the spinal cord and promote their differentiation into neural stem cells.关键词
补阳还五汤/骨髓间充质干细胞/脊髓损伤/气虚血瘀证/红核脊髓束Key words
Buyang Huanwu Decoction/bone marrow mesenchymal stem cell/spinal cord injury/Qi deficiency and blood stasis/rubrospinal tract分类
医药卫生引用本文复制引用
范瑜洁,聂颖,陈安,殷坚,杨奔,李亮..补阳还五汤对移植BMSCs在脊髓损伤局部存活、增殖及分化的影响[J].湖南中医药大学学报,2018,38(11):1321-1325,5.基金项目
国家自然科学基金资助项目(81302899) （81302899）
湖南省科技厅科技计划重点项目(2013SK2023) （2013SK2023）
湖南省中医药科研计划项目(2013111) （2013111）
湖南中医药大学中医诊断学国家重点学科开放基金(2014-37) （2014-37）
