昆明医科大学学报2018，Vol.39Issue(3)：55-58,4.
新型抗菌物质DAPC对变异链球菌粘附作用的影响
Effect of DAPC on Adhesion of Streptococcus Mutans
摘要
Abstract
Objective To analyze the property of the new antibacterial agent (N, N-di-n-decyl-N, N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate, DAPC) to prevent Streptococcus mutans' adhesion on tooth surface.Method determine the minimal inhibitory concentration and minimal bactericidal concentration of DAPC and Chlorhexidine gluconate by liquid dilution method.Set the Chlorhexidine gluconate as the positive control, while PBS as the negative control.Use the crystal violet staining to measure the quantity of biofilm.Re s ult The MIC of DAPC on Streptococcus mutans was 0.0031250％ and MBC was 0.0062500％. The MIC of Chlorhexidine gluconate was 0.0015625％, while the MBC was 0.0031250％.When concentration of the two antibacterial agents was MIC, the quantity of biofilm have no significance among three groups.However, when concentration of Chlorhexidine gluconate and DAPC were 0.12％ and 0.24％ respectively, biofilm of experimental group was lower than PBS, and there is no significance between Chlorhexidine gluconate group and DAPC group after 24 hours incubation.Conclus ion New antibacterial agent DAPC have significant effect in inhibiting of Streptococcus mutans.And the residual of DAPC on teeth can maintain a long time of antibacterial effect to inhibit Streptococcus mutans' adhesion.关键词
DAPC/葡萄糖酸氯己定/变异链球菌/粘附Key words
DAPC/Chlorhexidine gluconate/Streptococcus mutans/Adhesion分类
医药卫生引用本文复制引用
蒋娟娜,解保生,吴云鼎,周甜..新型抗菌物质DAPC对变异链球菌粘附作用的影响[J].昆明医科大学学报,2018,39(3):55-58,4.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(U0120140798) （U0120140798）
