兰州大学学报（医学版）2018，Vol.44Issue(4)：9-15,7.DOI:10.13885/j.issn.1000-2812.2018.04.002
ΔNp63α转录激活SOD2以及在细胞凋亡中的作用
ΔNp63α activates SOD2 expression and suppresses apoptosis
摘要
Abstract
Objective To elucidate the role of ΔNp63α in its regulation of manganese superoxide dismutase (SOD2) and suppression of apoptosis. Methods Human non-transformed mammary epithelial Michigan Cancer Foundation-10 A (MCF-10 A) cells tably expressing ΔNp63α were subjected to quantitative polymerase chain reaction (Q-PCR) and Western blot analyses as well as apoptosis assays for SOD2 and, under the condition of suspension growth of MCF-10 A cells. Results Overexpression of ΔNp63α transcriptionally activated SOD2 expression, resulting in reduced reactive oxygen species (ROS) levels and decreasing apoptosis under the condition of suspension growth of MCF-10 A. Conclusions ΔNp63α activates transcription of SOD2 and is concomitant with reduced apoptosis, suggesting a role for SOD2 in ΔNp63α-mediated tumorigenesis.关键词
ΔNp63α/锰超氧化物歧化酶/活性氧小分子/人永生化乳腺上皮细胞/细胞凋亡Key words
ΔNp63α/manganese superoxide dismutase/reactive oxygen species/michigan cancer foundation-10A/apoptosis分类
医药卫生引用本文复制引用
赵志强,曾朋,罗洪,肖智雄,张渝君,王阳..ΔNp63α转录激活SOD2以及在细胞凋亡中的作用[J].兰州大学学报（医学版）,2018,44(4):9-15,7.基金项目
国家自然科学基金项目(31170729) （31170729）
