兰州大学学报（医学版）2018，Vol.44Issue(4)：16-22,7.DOI:10.13885/j.issn.1000-2812.2018.04.003
微小RNA-30a在后发性白内障中对上皮间质转化的调控机制
Mechanism of miR-30a regulates epithelial-mesenchymal transition in posterior capsule opacification
摘要
Abstract
Objective To investigate into the role and regulation mechanism of microRNA (miR) -30 a in epithelial to mesenchymal transition (EMT) of posterior capsular cataract (PCO) . Methods The expression levels of miR-30 a and EMT markers (E-cadherin, Fibronectin) in 43 cases of PCO lens epithelium and 18 cases of normal lens epithelium were detected by real-time quantitative PCR. Pearson correlation coefficient was used to analyze the correlation between miR-30 a and EMT markers. The effect of overexpression of miR-30 a on the proliferation and invasion of human lens epithelial cell line SAR01/04, as well as the EMT-related protein expression were detected. The miR-30 a target gene was predicted by miRNA target gene prediction software, validated by the luciferase reporter assay. The overexpression of miR-30 a in PCO and normal lens epithelial primary cells was followed by detection of the differential expression of EMT-related proteins. Results miR30 a and E-cadherin were lowly expressed in PCO lens epithelial cells, while Fibronectin highly expressed.There was a significant positive correlation between miR-30 a and E-cadherin (r=0.754, P ＜ 0.001), but a negative correlation between miR-30 a and Fibronectin (r=-0.857, P ＜ 0.001) . Overexpression of miR-30 a reduced the proliferation and invasion ability of human lens epithelial cell line SAR01/04, followed by E-cadherin up-regulation, and Fibronectin, Slug, and Cox-2 down-regulation. Reporter gene assay revealed that miR-30 was capable of down-regulating the Slug expression by binding to the 3'-UTR sequence of the Slug gene. Overexpression of miR-30 a in PCO and normal lens epithelial cells could up-regulate E-cadherin expression and down-regulate the expression of Fibronectin, Slug, and Cox-2. Conclusions miR-30 a attenuates proliferation and invasion by inhibiting EMT in human lens epithelial cells by down-regulating Slug expression.关键词
后发性白内障/微小RNA-30a/上皮间质转化/调控Key words
posterior capsule opacification/miR-30a/epithelial-mesenchymal transition/regulation分类
医药卫生引用本文复制引用
马君择,柏凌,刘子瑶,范雅稚,权彦龙..微小RNA-30a在后发性白内障中对上皮间质转化的调控机制[J].兰州大学学报（医学版）,2018,44(4):16-22,7.基金项目
陕西省重点研究发展计划项目(2017SF-162) （2017SF-162）
