兰州大学学报（医学版）2018，Vol.44Issue(4)：27-32,6.DOI:10.13885/j.issn.1000-2812.2018.04.005
胆囊切除术对小鼠肝细胞膜转运体胆盐输出泵的影响
Effect of cholecystectomy on the expression of hepatobiliary membrane transporter bile salt export pump in mouse
摘要
Abstract
Objective To investigate into the effects of cholecystectomy on the expression of hepatobiliary membrane transporter Bsep and bile salts concentration in the liver and serum of mouse. Methods The cholecystectomy model was applied to the experiment, sampling blood was collected from eyes and hepastic tissue one, three, six weeks after operation; the concentrations of bile acid in liver and serum were determined by LC-MS/MS, the protein mass of Bsep was analyzed by Western blot and RT-qPCR. Results In contrast to the sham-operation, the vital signs did not show prominent changes, the expression of hepatobiliary membrane transporter Bsep was reduced (P ＜ 0.01) and the bile salts concentration in liver, including β-MCA, TCDCA, TDCA, TUDCA and THDCA, was increased and in serum, including β-MCA, CA, TCA, TCDCA and UD-CA, was reduced and part of them were changed observably (P ＜ 0.05) in the third week. Conclusions The cholecystectomy may cause the expression of the transporter Bsep to the effect that lead bile salts would be outwardly reduced. Meanwhile, the bile salts concentration and partition ratio in liver and serum of mouse may change at different levels, which could be associated with a postoperative bile duct calculi recurrence.关键词
胆囊切除术/胆酸盐/胆盐输出泵Key words
cholecystectomy/bile salts/bile salt export pump分类
医药卫生引用本文复制引用
王沛,杨玉全,张辉..胆囊切除术对小鼠肝细胞膜转运体胆盐输出泵的影响[J].兰州大学学报（医学版）,2018,44(4):27-32,6.基金项目
国家自然科学青年基金项目(81673508) （81673508）
甘肃省自然科学基金项目(1606RJZA139) （1606RJZA139）
兰州大学学生创新创业基金项目(20171073001511) （20171073001511）
