兰州大学学报（医学版）2018，Vol.44Issue(5)：37-43,7.DOI:10.13885/j.issn.1000-2812.2018.05.007
αA晶体蛋白在缺氧状态下对视网膜的血管内皮细胞血管生成作用
Effects of αA-crystallin on angiogenesis of retinal vascular endothelial cells under hypoxic condition
摘要
Abstract
Objective This study sought to treat a simian retinal vascular endothelial cell line RF/6 A with cobalt chloride (CoCl2), simulating a cellular hypoxic microenvironment. Then the effects of αA-crystallin (CRYAA) expression on the behavior of RF/6 A cells, in particular the endothelial cell angiogenesis under the hypoxic condition, was examined. Methods Real-time quantitative PCR was used to screen the small interfering RNA (siRNA) that could effectively inhibit CRYAA expression from three different siRNAs. The expression of CRYAA protein was examined by Western blotting analysis. The effects of knocking down CRYAA expression on RF/6 A cell's proliferative, migratory and tubulogenic capabilities induced by the hypoxia were examined by the cell counting kit-8, Transwell assay and Matrigel assay, respectively. Results CRYAA gene expression was up-regulated in the RF/6 A cells under the CoCl2-induced hypoxia (P ＜ 0.01) . However, only siRNA945 significantly inhibited the up-regulated expression of CRYAA (P ＜ 0.001) . Western blottingconfirmed a similar trend among the experimental groups of CRYAA protein as that of CRYAA transcript.The CoCl2-induced hypoxia enhanced the proliferation, migration and tube formation capabilities of RF/6 A cells, whereas siRNA945 significantly compromised these hypoxia-induced angiogenic capabilities of the retinal vascular endothelial cells (all P ＜ 0.001) . Conclusion Under the hypoxic microenvironment simulated by CoCl2, knocking down CRYAA profoundly inhibited the hypoxia-induced angiogenesis of retinal vascular endothelial cells.关键词
αA-晶体蛋白/细胞增殖/迁移/管腔成形/缺氧Key words
αA-crystallin cell/proliferation/migration/tube formation/hypoxia分类
医药卫生引用本文复制引用
郑嘉琳,吴绵绵,张琰,张红,孙靖,石怡..αA晶体蛋白在缺氧状态下对视网膜的血管内皮细胞血管生成作用[J].兰州大学学报（医学版）,2018,44(5):37-43,7.基金项目
天津市高等学校科技发展基金项目(20120129) （20120129）
