兰州大学学报（医学版）2018，Vol.44Issue(5)：44-48,5.DOI:10.13885/j.issn.1000-2812.2018.05.008
亲代同型半胱氨酸水平升高对子代脑组织及骨骼肌端粒长度的影响
Effects of elevated homocysteine levels in parental generation on the telomere length of offspring brain and skeletal muscles
摘要
Abstract
Objective To explore the effects of elevated homocysteine levels levels in parental generation on the telomere length of offspring brain and skeletal muscles. Methods 8 mice were randomly divided into experimental group and control group, each group consisting of 3 females and 1 male. The experimental group was fed with 1％ methionine and the control group with ordinary feed. 4 weeks later, female and male mice were mated respectively until the birth of their progeny, whose number was recorded.0.5 mL blood of the parental generation was retained, and the plasma Hcy concentration was measured by the enzyme-linked immunosorbent assay method. From each of the two groups, 3 mice were randomly selected from each female, forming a new experimental group (offspring) and a control group (offspring) with 9 rats in each. At the same time, 10 mg tissue from the parent and offspring's brain and the skeletal muscle of the hind limbs was taken and the real-time fluorescence quantitative polymerase chain reaction method was usedto determine the length of the telomere. Results Compared with the control group, the level of plasma Hcy in the parents increased significantly while the number of progenies decreased (P ＜ 0.05), but there was no significant difference in the telomere length of either the brain tissue or the skeletal muscle (P＞ 0.05) . The telomere length of the offspring brain tissue was significantly shorter than that of the control group (P ＜ 0.05), and the skeletal muscle tissue was positively correlated with the telomere length of the brain tissue (r = 0.727, P =0.041) . Conclusion The increase of Hcy level in parental plasma may have potential an adverse effect on the growth offspring through affecting the telomere length.关键词
同型半胱氨酸/端粒/骨骼肌/脑/小鼠Key words
homocysteine/telomere/skeletal muscle/brain/mice分类
医药卫生引用本文复制引用
王海生,付佳佳,张世杰,李昕,赵丽婷,刘洋..亲代同型半胱氨酸水平升高对子代脑组织及骨骼肌端粒长度的影响[J].兰州大学学报（医学版）,2018,44(5):44-48,5.基金项目
国家自然科学基金项目(11672332) （11672332）
天津市科技计划项目(16ZXHLSY00120) （16ZXHLSY00120）
