临床神经外科杂志2019，Vol.16Issue(4)：278-282,5.DOI:10.3969/j.issn.1672-7770.2019.04.001
miR-155在胶质瘤干细胞样细胞的表达及临床意义
The expression of miR-155 in GSCs and its clinical significance
摘要
Abstract
To study the different expression of miR-155 in glioblastoma stem-like cells(GSCs), neural stem cells ( NSCs) and different grades glioma.To clarify the relationship of miR-155 expression, degree of malignancy and prognosis of glioma patients.Methods GSCs and NSCs were isolated in vitro using neurosphere culture , then characteristics of the GSCs and NSCs were identified by experiments of immunoflurescence and xenograft.QRT PCR technology was used to detect the expression levels of miR-155 in GSCs, NSCs, gliomas of different grades and normal brain tissues.Kaplan-meier survival curve analysis was performed in combination with patient prognosis.Results The expression of miR-155 increased significantly in GSCs compared with NSCs (P＜0.05), and also increased with the malignant degree of glioma.The expression level of miR-155 in malignant glioma ( WHO Ⅲ,Ⅳ) was obviously higher than that of normal brain tissue (all P＜0.05).The high expression of miR-155 was closely related to the poor prognosis of glioma. Conclusion The expression of miR-155 elevates in GSCs and malignant glioma specimens , and can be considered as biomarker for diagnosis and prognosis judgment of glioma.关键词
miR-155/胶质瘤/胶质瘤干细胞样细胞/神经干细胞/预后Key words
miR-155/glioblastoma/glioblastoma stem-like cells/neural stem cells/prognosis of glioma分类
医药卫生引用本文复制引用
王瑞彤,闫昕,陈辉,赵明明,王淑为,刘爽,尹丰..miR-155在胶质瘤干细胞样细胞的表达及临床意义[J].临床神经外科杂志,2019,16(4):278-282,5.基金项目
国家自然科学基金(81172384) （81172384）
