临床神经外科杂志2019，Vol.16Issue(4)：283-289,7.DOI:10.3969/j.issn.1672-7770.2019.04.002
RNA结合蛋白RBM38表达对胶质瘤细胞增殖、迁移、侵袭能力的影响
Effect of RNA-binding motif protein 38 on proliferation, migration and invasion of glioma cells
摘要
Abstract
To investigate the expression level of RNA-binding motif protein 38 (RBM38) in glioma tissues and cells , and to identify its effects on the proliferation , migration, invasion and apoptosis of glioma cells.Methods RBM38 mRNA levels in glioma tissues and normal adjacent tissues were analyzed using qRT-PCR(quantitative real-time PCR).RBM38 levels in glioblastoma cell line U87, U251and human astrocyte HA1800 cells were detected using qRT-PCR and Western Blot.Additionally, the correlation between RBM38 expression in glioma tissues and the corresponding clinical pathological characteristics of the patients was explored , based on the IHC results of RMB38 expression in 59 human glioma tissues.Specific small interfering RNAs targeting RBM38 were transfected into glioma cell lines U87 and U251 using liposome-mediated transfection.The effects of RBM38 on the proliferation, migration, invasion and apoptosis of glioma cells were examined by CCK-8 proliferation assay , scratch assay , transwell chamber assay and flow cytometry assay respectively.The qRT-PCR was conducted to detect the P53 expression in the RBM38-depleted glioma cells.Results The expression of RBM38 in glioma tissues was significantly higher , in comparison with that of normal adjacent tissues ( P＜0.05).RBM38 levels were significantly up-regulated in glioma cell , compared with those in normal cells (all P＜0.001). Immunohistochemical results indicated RBM 38 expression was higher in high-grade gliomas compared with low-grade gliomas ( P ＜0.05 ).The inhibition of RBM38 expression resulted in significantly reduced proliferation rate of U 87 and U251 cells(P＜0.05), suppressed migration and invasion capacity of glioma cells ( P＜0.05), and significantly enhanced apoptosis rate of glioma cells(P ＜0.05 ).The expression level of P53 gene was significantly increased in the RBM 38-depleted glioma cells(P＜0.05).Conclusions RBM38 expression is remarkably high in glioma tissues and cell lines.The high expression level of RBM38 is closely related to the clinical grade of glioma.Down-regulation of RBM38 can inhibit the proliferation, migration, invasion of glioma cells, promote cellular apoptosis , and improve the expression of P53 gene.The data demonstrate that RBM38 can be used as novel target for the clinical diagnosis and intervention of glioma.关键词
RNA结合蛋白/RNA结合基序蛋白38/胶质瘤/细胞增殖/细胞迁移Key words
RNA-binding protein/RNA-binding motif protein 38/glioma/cell proliferation/cell migration分类
医药卫生引用本文复制引用
郭中叶,蒋世一,邱云,廖克曼,侯鹏,鲁晓杰..RNA结合蛋白RBM38表达对胶质瘤细胞增殖、迁移、侵袭能力的影响[J].临床神经外科杂志,2019,16(4):283-289,7.基金项目
江苏省省级科技专项资金(BL2013006) （BL2013006）
