临床神经外科杂志2019，Vol.16Issue(4)：295-298,303,5.DOI:10.3969/j.issn.1672-7770.2019.04.004
下调CRNDE基因表达对MAPK/ERK通路中相关基因的影响
Effect of down-regulated LncRNA CRNDE expression in MAPK／ERK pathway
摘要
Abstract
To investigate the effect of down-regulated LncRNA CRNDE expression for key kinases and transcription factors in the MAPK／ERK pathway.Methods The LncRNA CRNDE in U251 cells were interfered by using SiRNA.The non-transfected and control group were set up.By Real Time PCR method, the effect of interfering LncRNA CRNDE on U251 cell for key kinases and transcription factors in the MAPK／ERK pathway was observed.Results Compared to the non-transfected group and control group , the expression of the key kinases ( Raf-1, MEK2, ERK1, ERK2 ) and transcription factors ( c-Myc, NF-κB ) in MAPK／ERK pathway were down-regulated for siRNA group.There were significant differences between non-transfected group, control group and siRNA group ( all P ＜0.05).Conclusion CRNDE can enhance the abilities of cell proliferation and migration, inhibit the ability of cell apoptosis in glioma through activating related gene expression in MAPK／ERK pathway.关键词
结直肠癌差异表达长链非编码RNA/MAPK/ERK通路/增殖/凋亡/迁移Key words
CRNDE/MAPK/ERK pathway/proliferation/apoptosis/migration分类
生物科学引用本文复制引用
邓文月,杜浩,何远志,黄玲玥,路营营,汤伟,景世元..下调CRNDE基因表达对MAPK/ERK通路中相关基因的影响[J].临床神经外科杂志,2019,16(4):295-298,303,5.基金项目
武汉市卫生计生科研基金(WX17Q27) （WX17Q27）
