肿瘤预防与治疗2019，Vol.32Issue(6)：480-485,6.DOI:10. 3969/j. issn. 1674-0904. 2019. 06. 002
ADH1B对鼻咽癌细胞的增殖及迁移能力的影响*
Effect of ADH1B on Proliferation and Migration of Nasopharyngeal Carcinoma Cells
摘要
Abstract
Nasopharyngeal carcinoma (NPC) has become the main cause of death in head and neck cancer in China. Failed local treatment and distant metastasis are the main causes of poor prognosis in patients with advanced NPC. A large number of studies have shown that ADHlB is a risk gene for a variety of cancers. This study investigated the differential expression of ADHlB in NPC cells and its effect on the proliferation and migration of NPC cells. Methods: Bioinformatics analysis was performed to compare the relative expression of ADHlB in head and neck squamous cell carcinoma (HNSC) and normal tissue. Quantitative real-time PCR (qRT-PCR) was used to detect the mRNA expression level of ADHlB in NP69, a normal immortalized nasopharyngeal epithelial cell, and in CNE-1, CNE-2, 5-8F and 6-10B, NPC cells. On this basis, a stable NPC cell line which highly expresses ADHlB and a pCHD empty vector control was established. The growth curve experiment was used to test the proliferative ability of NPC cells, and the scratch wound healing assay was used to test the migration ability of NPC cells. Results: Bioinformatics analysis showed that ADHlB expression in HNSC was lower than that in normal tissue. ADHlB expression was lower in CNE-1, CNE-2, 5-8F, and 6-10B than in NP69 cells. The proliferation of NPC cells in the over-expressed ADHlB group was lower than that in the pCDH group ( P<0. 05), and the wound healing rate in the former was lower than that in the latter (P<0. 05). Conclusion: The expression of ADHlB in NPC cells was significantly lower than that in normal nasopharyngeal epithelial cells, which was consistent with results of bioinformatics anal-ysis. The high expression of ADHlB in NPC affects the proliferation and migration of NPC cells, which is closely related to and may be an important target for the occurrence and development of NPC.关键词
ADHlB/鼻咽癌细胞/增殖/迁移Key words
ADHlB/Nasopharyngeal carcinoma cells/Proliferation/ Migration分类
医药卫生引用本文复制引用
吴梦鸽,张睿,刘芮吟,费继敏,杨翠萍..ADH1B对鼻咽癌细胞的增殖及迁移能力的影响*[J].肿瘤预防与治疗,2019,32(6):480-485,6.基金项目
云南省卫计委高层次卫生技术人才培养项目(编号:D-201659) This study was supported by Foundation for High-Level Technical Talents Training Project of the Health Planning Commission of Yunnan Province ( NO. D-201659) （编号:D-201659）
