实验动物与比较医学Issue(1)：10-15,6.DOI:10.3969/j.issn.1674-5817.2018.01.002
应用微卫星标记对两个SD大鼠封闭群的遗传分析
Genetic Analysis on Two Closed Colonies of SD Rats Using Microsatellite DNA Markers
摘要
Abstract
Objective To analyze genetic diversity and relationship of two closed colonies of SD rats from Beijing and Shanghai respectively. Methods Seven microsatellite loci were tested by HardyWeinberg equilibrium (Hardy-Weinberg equilibrium, HWE), meanwhile, the values of the allele frequency, gene heterozygosity, polymorphic information content (PIC), the genetic distance and some other indexes were counted. Results The average number of observed alleles are 4.857 1 and 5.142 9 in two colonies respectively. The average observed heterozygosity were 0.647 6 and 0.585 7, the average PIC were 0.644 5, 0.667 6 respectively. Three and two loci showed evident deviation from that of the HWE (P＜0.05) in SD rat colony from Beijing and Shanghai respectively. The unbiased genetic distance of the two colonies was 0.158 2 (Nei 1978). Conclusion The genetic diversity of SD rat colonies from Beijing and Shanghai are relatively rich. There are medium level of genetic differentiation between the two colonies. Microsatellite marker technique is an effective mean for genetic monitoring for closed clolony of SD rats.关键词
SD大鼠/微卫星/封闭群/遗传分析Key words
SD rat/Microsatellite DNA/Closed colony/Genetic analysis分类
生物科学引用本文复制引用
洪杨,袁明铭,周静,张磊,董涵..应用微卫星标记对两个SD大鼠封闭群的遗传分析[J].实验动物与比较医学,2018,(1):10-15,6.基金项目
四川省基本科研业务专项课题项目(A-2015N-16) （A-2015N-16）
四川省基本科研业务专项课题项目(2016ysky0074) （2016ysky0074）
四川省科技基础条件平台项目-四川省实验动物共享服务平台建设(2010TJPT0010) （2010TJPT0010）
