实验动物与比较医学Issue(1)：22-28,7.DOI:10.3969/j.issn.1674-5817.2018.01.004
一种体内实时观察黑色素瘤的斑马鱼模型
A Melanoma Zebrafish Model for Real-time Imaging in vivo
摘要
Abstract
Objective To establish a zebrafish model for real-time monotoring the development of melanoma in vivo and screening relative drugs. Methods The expression effect of p MITFa1-Red V12/h Ras plasmid, in which the melanoma was labeled by red fluorescent protein (RFP), was verified in mouse B16 melanocytes. After microinjecting p MITFa1-Red V12/h Ras into 1-cell stage of fertilized eggs of zebrafish, we monitored the process of melanoma formation with in vivo imaging system in zebrafish.Results p MITFa1-Red V12/h Ras was successfully expressed in mouse melanoma B16 cells. Three days after microinjection of p MITFa1-Red V12/h Ras vector to 1-cell satge of zebrafish, melanomas labeled by RFP protein was observed under the fluorescence microscope. Eight weeks later, melanomas were observed clearly by eyes. The rate of tumor formation was 100％. When microinjected into p MITFa1-Red V12/h Ras vector into fertilized eggs of TG (zlyz:EGFP) transgenic zebrafish, the interaction between the melanoma cells labeled by RFP protein and the macrophages labeled by green fluorescent protein was clearly observed in in vivo imaging system. Conclusion Successfully established a zebrafish model for real-time monitoring melanoma in vivo, which is cheap and easy to operate.关键词
黑色素瘤模型/斑马鱼/实时观察Key words
Melanoma model/Zebrafish/Real-time imaging分类
生物科学引用本文复制引用
周斌,盛哲津,冯琛卓,李利妹..一种体内实时观察黑色素瘤的斑马鱼模型[J].实验动物与比较医学,2018,(1):22-28,7.基金项目
中央高校基础科研业务专项基金(No.1507219069) （No.1507219069）
浙江省自然科学基金一般项目(No.LY18H090009) （No.LY18H090009）
浦东新区卫生计生委科技项目(No.PW2017B-7) （No.PW2017B-7）
