实验动物与比较医学Issue(1)：36-39,4.DOI:10.3969/j.issn.1674-5817.2018.01.006
嗜肠型小鼠肝炎病毒RT-PCR诊断方法的建立及其应用
Establishment and Application of RT-PCR Diagnostic Method for Enterotropic Mouse Hepatitis Virus
摘要
Abstract
Objective To establish a method for RT-PCR detection of mouse hepatitis virus (MHV) and provide a rapid method for monitoring the daily production process of mice. Methods The total RNA form mouse colon tissue was extracted. The first strand c DNA was synthesized by reverse transcription reaction. Then using the c DNA as the template, a DNA fragment of MHV was amplified by PCR with four pairs of specific primers. The PCR reaction conditions (including primers, primer concentration, annealing temperature, template concentration, etc.) were optimized. Results The two pairs of MHV primers were effective and specific. The PCR results of these two pairs of primers were consistent with the results of ELISA. Conclusion The RT-PCR detection method of MHV was specific and relatively simple. It can be used as a rapid method for MHV detection in the production process of mice.关键词
小鼠肝炎病毒(MHV)/RT-PCR/诊断方法Key words
Mouse hepatitis virus (MHV)/RT-PCR/Diagnostic methods分类
生物科学引用本文复制引用
艾东旭,孙菲,李钰,法云智,孙兆增,李莲瑞..嗜肠型小鼠肝炎病毒RT-PCR诊断方法的建立及其应用[J].实验动物与比较医学,2018,(1):36-39,4.基金项目
军队实验动物专项[SYDW(2014)006] （2014）
