实验动物与比较医学Issue(6)：1-9,9.DOI:10.3969/j.issn.1674-5817.2018.01.001
封闭群树鼩的微卫星遗传特性分析
Analysis of Microsatellite Genetic Characteristics in of Closed Colony of Tree Shrews
摘要
Abstract
Objective To find out the genetic variation and differentiation of the IIV generations in tree shrews (Tupaia belangeri chinensis), and provide genetic bases for closed colony tree shrews.Methods The forty-nine genomic DNA of blood samples were extracted from IIV generations tree shrews, the amplification products were detected by capillary electrophoresis and marked with fluorescent tags (FAM) PCR, then the genetic diversity of the tree shrew colony was analyzed comparatively using POPGENE, FSTAT software with tree shrews 18 microsatellite loci. Results The 89 alleles were found in the tree shrew colony, the average effective number of alleles, observed heterozygosity, expected heterozygosity, polymorphism information content, Shannon information index and allele richness were 3.779, 0.523, 0.613, 0.558, 1.277 and 2.938 respectively. The mean value of Fst was 0.046 in the colony. The genetic distance and unbiased genetic distance were 0.0490.159 and 0.0220.109 respectively. Conclusions The closed clony tree shrew shows high genetic diversity, and there was no significant difference in genetic diversity and differentiation.关键词
滇西亚种树鼩/封闭群/微卫星位点/遗传多样性/遗传管理Key words
Tree shrew (Tupaia belangeri chinensis)/Closed colony/Microsatellite loci/Genetic diversity/Genetic management分类
生物科学引用本文复制引用
刘城秀,李娜,仝品芬,王文广,陆彩霞,罕园园,匡德宣,孙晓梅,代解杰..封闭群树鼩的微卫星遗传特性分析[J].实验动物与比较医学,2018,(6):1-9,9.基金项目
国家科技支撑计划项目(2014BAI01B00) （2014BAI01B00）
云南省科技人才和平台计划项目(2017HC019) （2017HC019）
云南省重大科技专项(2017ZF007) （2017ZF007）
