实验动物与比较医学Issue(6)：16-21,6.DOI:10.3969/j.issn.1674-5817.2018.01.003
单核苷酸多态性位点法对国内近交系小鼠遗传检测
Genetic Mornitoring for Inbred Mice with Single Nucleotide Polymorphisms
摘要
Abstract
Objective To detect the genetic quality of 29 inbred strains mice from 36 different colonies using 95 single nucleotide polymorphism (SNP) loci and analyze the different genotype among different strains. Methods All genotypes of samples from domestic including 36 inbred mice colonies such as C57BL/6J, BALB/c were identified by the methods of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using 95 SNPs referenced from domestic and international literature. Then the numbers of loci with different genotypes between each two strains were analyzed and compared. Results Totally 29 colonies (80.56％) of the mouse were successfully genotyped with 95 SNPs while very few strains weren't, because of sample's quality focusing on 3strains with the background of BALB/c. Most loci were homozygous and the rate of monomorphic loci within strains was up to 98.95％. The numbers of loci with different genotypes between each two colonies reached maximal 58 loci and minimal 1 locus which distributed in the same strain from distinct sources and the gene modified lines between their backgrounds. Conclusions The selected SNPs could be applied to mornitor and identify the genetic condition of inbred mice. However, SNP loci panel should be optimized on behalf of each strain's characteristics.关键词
近交系小鼠/单核苷酸多态性(SNP)/基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)/遗传检测Key words
Inbred mice/Single nucleotide polymorphism (SNP)/Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS)/Genetic mornitoring分类
生物科学引用本文复制引用
李银银,陈振文,刘丽均,李长龙,刘欣,吕建祎,郭萌,杜小燕..单核苷酸多态性位点法对国内近交系小鼠遗传检测[J].实验动物与比较医学,2018,(6):16-21,6.基金项目
国家科技支撑计划(No.2015BAI07B01) （No.2015BAI07B01）
国家重点研发计划(No.2017YFD0501602) （No.2017YFD0501602）
