生态学杂志2018，Vol.37Issue(8)：2331-2338,8.DOI:10.13292/j.1000-4890.201808.030
氮营养对苗期菘蓝生长及活性成分的影响
Effects of nitrogen nutrition on the growth and active components of Isatis indigotica Fort.seedlings
摘要
Abstract
To study the effects of nitrogen (N) nutrition on the growth and active components of Isatis indigotica Fort., five N levels (0, 2. 5, 5, 10, 15 mmol · L-1, designated as N0, N1, N2, N3, N4 respectively) were applied in a pot experiment in greenhouse. The growth characteristics, photosynthesis parameters, N assimilation contents, N metabolism enzyme activities, the contents of indigo, indirubin, and total flavonoid in leaves and epigoitrin content in roots were measured. The results showed that plant height, main root diameter, and dry biomass of I. indigotica seedlings significantly decreased and root-shoot ratio increased under N0-N3 treatments.With the decreases of N level, the chlorophyll content, carotenoid content, net photosynthetic rate, transpiration rate and stomatal conductance of leaves gradually decreased, while the intercellular carbon dioxide concentration increased. The contents of soluble protein, free amino acid, and nitric N in leaves were decreased, and nitrate reductase activity and glutamine synthase activity were inhibited under low N nutrition. The contents of indirubin and total flavonoid in leaves under N0-N2 treatments were at higher levels. In contrast, contents of indigo in leaves were decreased significantly under low N nutrition and epigoitrin contents in roots were the lowest at N0 treatment. In conclusion, the growth and partial physiological characteristics of I. indigotica seedlings were significantly influenced by low N levels. The contents of indigo in leaves and epigoitrin contents in roots were declined under low N levels, while the accumulations of indigo and total flavonoid were promoted. Considering the biomass and active component contents, the yield and active components will show good performance under the N application levels of 10-15 mmol·L-1.关键词
菘蓝/氮营养/光合特性/氮代谢/活性成分Key words
Isatis indigotica Fort./nitrogen nutrition/photosynthetic characteristics/nitrogen metabolism/active component引用本文复制引用
关佳莉,王刚,陈曦,张梦蕊,缪雨静,唐晓清..氮营养对苗期菘蓝生长及活性成分的影响[J].生态学杂志,2018,37(8):2331-2338,8.基金项目
国家自然科学基金项目(31171486) （31171486）
南京农业大学校级大学生实践创新训练项目(1714A15)资助 （1714A15）
