烟草科技2019，Vol.52Issue(6)：16-22,7.DOI:10.16135/j.issn1002-0861.2018.0400
利用VIGS技术研究NtbHLH93基因在烟草甾醇代谢中的功能
Study on function of NtbHLH93 in sterol metabolism of tobacco by VIGS
摘要
Abstract
To study the function of NtbHLH93, virus-induced gene silencing (VIGS) was used to downregulate NtbHLH93 expression in tobacco. The results showed that when NtbHLH93 expression was downregulated, the contents of total sterol, stigmasterol, cholesterol and β-sitosterol significantly decreased in pTRV2-NtbHLH93 tobacco seedlings. Transcriptome sequencing revealed that there were 259 differentially expressed genes in NtbHLH93 expression downregulated tobacco seedlings. KEGG pathway analysis indicated that two genes (Niben101Scf00063g13014.1 and Niben101Scf06249g03002.1) were upregulated in MEP (2-C-methyl-D-erythritol-4-phosphate) pathway, a terpenoid metabolism pathway in plastids. The sequences of the two genes were highly similar to geranylgeranyl diphosphate synthases (GGPPS) gene in MEP pathway, which suggested that the upregulation of Niben101Scf00063g13014.1 and Niben101Scf06249g03002.1 might relate to the inhibition of sterol synthesis.关键词
烟草/bHLH93/甾醇/VIGS技术Key words
Tobacco/bHLH93/Sterol/VIGS分类
轻工纺织引用本文复制引用
武明珠,罗朝鹏,曹培健,杨军,刘瑞霞,王中,郑庆霞,张剑锋,李泽锋,谢小东,魏攀,李锋..利用VIGS技术研究NtbHLH93基因在烟草甾醇代谢中的功能[J].烟草科技,2019,52(6):16-22,7.基金项目
国家自然科学基金青年基金项目"植物激素调控绿原酸合成关键基因HQT分子机制研究"(31701347). （31701347）
