应用生态学报2018，Vol.29Issue(3)：899-908,10.DOI:10.13287/j.1001-9332.201803.024
外源表油菜素内酯对NaHCO3胁迫下黄瓜幼苗生长及氧化还原平衡的影响
Effects of exogenous 2,4-epibrassinolide on the growth and redox balance of cucumber seedlings under NaHCO3 stress
摘要
Abstract
The effects of 0.2 μmol·L-1 exogenous 2,4-epibrassinolide (EBR) on the growth and reactive oxygen species metabolism of cucumber seedlings('Jinyan 4'cucumber) under salt-alkaline stress (30 mmol·L-1 NaHCO3) were examined by hydroponics method. The results showed that NaHCO3 stress significantly induced production of O2-· and accumulation of H2O2 in leaves and roots,resulting in the increases of MDA content and electrolyte leakage. Under NaHCO3 stress,activities of superoxide dismutase,peroxidase,catalase,ascorbate peroxidase,dehydroascorbate reductase,monodehydrodroascorbate reductase,and glutathione reductase as well as contents of ascorbic acid and glutathione were firstly increased and then decreased with progress of stress time. Exogenous EBR application significantly increased the activities of antioxidant enzymes and contents of antioxidants as well as the ratio of AsA/DHA (dehydroascorbic acid) and GSH/GSSG (L-glutathione oxidized) in leaves and roots of cucumber under NaHCO3 stress. Such changes improved the redox hemostasis in plants,reduced the level of reactive oxygen species,and alleviated the membrane lipid peroxidation. Together,they increased the alkaline tolerance of cucumber seedlings.关键词
黄瓜/盐碱胁迫/活性氧/抗氧化/AsA-GSH循环Key words
cucumber/salt-alkaline stress/reactive oxygen species/antioxidation/AsA-GSH cycle引用本文复制引用
聂文婧,王硕硕,荆鑫,巩彪,魏珉,杨凤娟,李岩,史庆华..外源表油菜素内酯对NaHCO3胁迫下黄瓜幼苗生长及氧化还原平衡的影响[J].应用生态学报,2018,29(3):899-908,10.基金项目
本文由国家自然科学基金项目(31372059)、山东省现代农业产业技术体系项目(SDAIT-05-10)和山东省农业科学院农业科技创新工程项目(CXGC2016B06)资助 （31372059）
