中国临床药理学与治疗学Issue(7)：721-727,7.DOI:10.12092/j.issn.1009-2501.2018.07.001
二氢杨梅素防治磨损颗粒诱导的小鼠颅骨骨溶解的实验研究
Effects of dihydromyricetin on wear-particle-induced osteolysis in murine calvarial model
摘要
Abstract
AIM:To investigate the effects of dihydromyricetin (DMY) on wear-particle-induced osteolysis in murine calvarial model.METHODS:Thirty male C57 BL/J6 mice were randomly divided into the sham group, the control group, and the DMY group (10 in each group).Except for the mice from the sham group, mice from the control group and DMY group received 30 mg Ti particles into calvaria to induce osteolysis in murine calvarial model.Mice from the DMY group were gavage-fed daily with DMY of 500 mg/kg, while mice from the sham group and control group were gavage-fed daily with saline.Calvaria and vein blood samples from each group were harvested for further micro-CT and pathological analysis at the 14 th after the surgery.RESULTS:Micro-CT showed that the severity of osteolysis was significantly reduced in DMY group.And the BMD of the samples in DMY group was significantly higher than that of the control group (P ＜0.05).By the HE and TRAP staining analysis, less osteoclasts were found in the samples in DMY group.In addition, the application of DMY also decreased the serum TNF-α, IL-1β, and RANKL level yet improved OPG level as compared with the positive control group (P ＜ 0.05).DMY could significantly inhibit the differentiation of RANKL-induced osteoclast by in vitro cell culture experiments.CONCLUSION:DMY can inhibit the wear-particle-induced osteolysis in murine calvarial model.关键词
小鼠/二氢杨梅素/钛颗粒/破骨细胞/骨溶解Key words
mouse/dihydromyricetin/Ti particles/osteoclast/osteolysis分类
医药卫生引用本文复制引用
黄东辉,郑杭生..二氢杨梅素防治磨损颗粒诱导的小鼠颅骨骨溶解的实验研究[J].中国临床药理学与治疗学,2018,(7):721-727,7.基金项目
国家自然科学基金(30873276) （30873276）
