中国临床药理学与治疗学Issue(7)：728-733,6.DOI:10.12092/j.issn.1009-2501.2018.07.002
蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究
Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line
摘要
Abstract
AIM:To investigate the radio-sensitivity effect of bufalin in Kyse150 cell line.METHODS:CCK-8 method was used to determine the IC50 value of bufalin in Kyse150 cell line.The linearquadratic (L-Q) curve was fitted according to the results of cloning formation and calculated the survival fraction of 2 Gy (SF2) and sensitization enhancement ratio (SER).Annexin V-FITC/PI double staining and Western blot were used to detect the effect of bufalin on apoptosis of esophageal cancer Kyse150 cell line.The effect of different bufalin doses on Kyse150 cell line radiosenstivity was detected by cloning formation assay.RESULTS:CCK-8 results showed that the IC50 value of bufalin in the Kyse150 cell line was 129.1 nmol/L (95％ CI:105.2-158.6).The L-Q curve fitted by colony formation assay showed that bufalin can significantly reduce the colony forming efficiency of cells after radiotherapy, and the sensitization enhancement ratio of bufalin in Kyse150 cell line was 1.463.Annexin V-FITC/PI double staining and Western blot results showed that bufalin can improve radiation-induced apoptosis, and showed that bufalin has a dose-dependent effect on radiosensitization of esophageal cancer Kyse150 cell line.CONCLUSION:Bufalin can enhance the radiosensitivity effect of the Kyse150 cell line in a dose-dependent manner.关键词
蟾毒灵/食管癌/放疗敏感性/线性二次模型/放疗增敏比Key words
bufalin/esophageal cancer/radiosensitivity/linear quadratic model/sensitization enhancement ratio分类
医药卫生引用本文复制引用
方为扬,李越,盛蕾,崔珍珍,仲飞,童铸廷..蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究[J].中国临床药理学与治疗学,2018,(7):728-733,6.基金项目
国家自然科学基金(81201743) （81201743）
中国教育部博士基金(20123420120008) （20123420120008）
安徽省博士后科学基金资助项目(2015B053) （2015B053）
安徽省高等学校省级质量工程大学生创新训练项目(201510366065) （201510366065）
