中国临床药理学与治疗学Issue(7)：749-754,6.DOI:10.12092/j.issn.1009-2501.2018.07.005
miR-150对药物代谢酶CYP3A4的调控作用
Regulative effects of miR-150 on CYP3A4
摘要
Abstract
AIM:To explore the role of mi R-150 in the regulation of CYP3 A4 and the underlying mechanism.METHODS:CYP3 A4 m RNA and protein expression were tested by RT-PCR and Western blot when the Chang liver cells were stimulated by FFA containing fatty acids free 1％ BSA.Bioinformatics mi RNA databases were used to identify CYP3 A4-related mi RNAs and the wild-type CYP3 A43'-UTR plasmids were constructed, mi R-150 and reporter plasmid were co-transfected into the Chang liver cells to test the expression ratio of the reported fluorescence and the correction fluorescence.Then the mi R-150 mimic were transfected into the Chang liver cells, while mi RNA inhibitor were transfected into the steatosis cells.RT-PCR and Western blot were used to detect CYP3 A4 m RNA and protein expression.RESULTS:The CYP3 A4 m RNA and protein level were statistically significantly decreased (P＜ 0.05), while the mature mi R-150 levels were increased (P ＜ 0.05) when the Chang liver cells were stimulated after 24 h by 1 mmol/L FFA.CYP3 A4 was found to be the target gene of mi R-150 with a bioinformatics analysis.Then CYP3 A4 m RNA level was significantly decreased (P ＜ 0.05).Meanwhile, the expression level of CYP3 A4 protein also decreased when the mi R-150 mimic were transfected into the Chang liver cells.But CYP3 A4 m RNA expression has no statistical significance (P = 0.071) in mi R-150 inhibitor group.CONCLUSION:The mi R-150 can combine with CYP3 A4 3'-UTR directly, and can further regulate the expression of CYP3 A4 m RNA.关键词
miR-150/CYP3A4/调控作用Key words
miR-150/CYP3A4/regulation分类
医药卫生引用本文复制引用
刘莉,彭金富,郭成贤,阳喜定,李海刚,阳国平..miR-150对药物代谢酶CYP3A4的调控作用[J].中国临床药理学与治疗学,2018,(7):749-754,6.基金项目
湖南省教育厅科学研究项目资助(17C0169) （17C0169）
湖南省教育厅科学研究项目资助(15C0160) （15C0160）
国家自然科学基金(81373476) （81373476）
新型药物制剂研发湖南省重点实验室培育基地资助(2016TP1029) （2016TP1029）
