中国临床药理学与治疗学Issue(7)：755-760,6.DOI:10.12092/j.issn.1009-2501.2018.07.006
CD40调节胰腺癌细胞生物学行为的研究
Effects of CD40 pathway on the biological behavior of pancreatic cancer cells
摘要
Abstract
AIM:To study the effect of CD40 L on the biological characteristics and stenness of pancreatic cancer cells.METHODS:The effect of different concentrations of s CD40 L (0, 1, 2, 4 μg/m L) on the proliferation of human pancreatic cancer cell line panc02 was analyzed by CCK-8 live cell counting assay.Apoptosis was detected by Annexin V/PI double staining;migrational ability was detected by Cell Scratch Assays.Q-PCR was used to detect the levels of c-Myc, OCT-4 and Sox-2 after panc02 treatment for 4 h/m L s CD40 L.A Balb/c nude mouse model of pancreatic cancer was established and randomly divided into two groups:control group (normal saline) and s CD40 L (10 mg/kg) group.They were injected intraperitoneally with s CD40 L 10 mg/kg and an equal volume of saline for three times a day.Tumor volume was measured using vernier calipers at 0, 7 th, 14 th, 21 st, and28 th day.RESULTS:Compared with the control group, the growth and migration of panc02 decreased significantly after s CD40 L treatment (1, 2, 4 μg/m L for 96 h), and apoptosis was promoted in a dose-dependent manner (P ＜ 0.01).s CD40 L (4μg/m L) significantly inhibited the expression of cMyc, OCT-4, Sox-2 of panc02 (P ＜ 0.01).In the nude mice tumor-bearing experiment, compared with the control group, s CD40 L significantly inhibited the tumor volume at 28 th day (P ＜ 0.01, 252 ± 13 vs.189 ± 9).CONCLUSION:Activation of CD40 pathway inhibits proliferation, migration and apoptosis of pancreatic cancer cells as well as the proliferation of pancreatic cancer cell lines in vivo.This effect may be related to the change of stemness in pancreatic cancer cell lines.关键词
胰腺癌/体内外增殖/CD40/CD40L/干性Key words
pancreatic cancer/proliferation in vivo and in vitro/CD40/CD40L/stemness分类
医药卫生引用本文复制引用
张桂枫,蔡加琴,崔同建,刘振华,许志贤..CD40调节胰腺癌细胞生物学行为的研究[J].中国临床药理学与治疗学,2018,(7):755-760,6.基金项目
福建省卫生计生青年科研课题(2013037) （2013037）
福建医科大学启航基金项目计划(2016QH106) （2016QH106）
