中国临床药理学与治疗学Issue(7)：761-769,9.DOI:10.12092/j.issn.1009-2501.2018.07.007
大鼠胃癌前病变形成过程中胃黏膜Sonic Hedgehog信号通路的变化
Changes of Sonic Hedgehog signaling pathway in the stomach mucosa of rats in the formation process of gastric precancerous lesions
摘要
Abstract
AIM:To investigate the changes of Sonic Hedgehog signaling pathway in the stomach mucosa of rats in the formation process of gastric precancerous lesions.METHODS:Seventy-two suckling rats in half genders were randomly and equally divided into normal group and model group.The rats in the model group were given 0.1 m L MNNG at the dosage of 800 mg/L by gavage per day for 10 days, while the rats in the normal group were given normal saline.Twelve rats in each group were sacrificed at the end of the 10 th, 22 th and 34 th week, respectively.Histopathologic changes of the gastric mucosa were observed by HE staining;the levels of Shh, Ptch1, Smo, Gli1, Gli2, Gli3, SuFu, Cyclin D1, Cyclin E1, c-Myc and β-actin m RNA in the gastric mucosa were determined by RealtimePCR;the expressions of Shh, Ptch1, Smo, Gli1, Su Fu, Cyclin D1, Cyclin E1, c-Myc, p-c-Myc protein were detected by Western Blot.RESULTS:With the development of the atrophy and dysplasia of gastric mucosa, the levels of Shh, Smo, Gli1, Cyclin D1, Cyclin E1 and c-Myc m RNA increased and those of Ptch1 and Su Fu m RNA decreased;the expressions of Shh, Smo, Gli1, Cyclin D1, Cyclin E1, p-c-Myc protein showed an increase trend while the expressions of Ptch1 and Su Fu protein decreased.CONCLUSION:Shh signaling is activated in the formation process of gastric precancerous lesions, which indicates Shh signaling pathway participates in the development and progression of gastric precancerous lesions.关键词
1-甲基-3-硝基-1-亚硝基胍(MNNG)/胃癌前病变/Sonic Hedgehog信号通路Key words
MNNG/precancerous lesions of gastric cancer/Sonic Hedgehog signaling pathway分类
医药卫生引用本文复制引用
刘庆生,俞建顺,蔡丹莉,严茂祥,王小奇,桑怡..大鼠胃癌前病变形成过程中胃黏膜Sonic Hedgehog信号通路的变化[J].中国临床药理学与治疗学,2018,(7):761-769,9.基金项目
浙江省自然科学基金(LY13H290004) （LY13H290004）
杭州市科技计划项目(20130733Q18) （20130733Q18）
杭州市卫生科技计划重点项目(2016Z09) （2016Z09）
