中国中医药信息杂志2018，Vol.25Issue(9)：44-48,5.DOI:10.3969/j.issn.1005-5304.2018.09.011
左归降糖解郁方对糖尿病并发抑郁症大鼠海马神经元病理形态及凋亡的影响
Effects of Zuogui Jiangtang Jieyu Prescription on Pathological Morphology and Apoptosis of Hippocampal Neurons in Rats of Diabetes Mellitus with Depression
摘要
Abstract
Objective To observe the effects of Zuogui Jiangtang Jieyu Prescription (ZGJTJYP) on pathological morphology of hippocampal neurons in rats of diabetes mellitus with depression (DD); To discuss its protective mechanism of action. Methods DD rat model was established by tail vein injection of STZ combined with chronic stress. Totally seventy-two SPF rats were randomly divided into normal group, model group, positive medicine group, ZGJTJYP low-, medium-, and high-dose groups, with 12 cases in each group, and were given corresponding medicine. After the last administration, blood glucose and behavioral changes were measured in each group. HE staining, Nissl staining, and Golgi staining were used to observe the changes of pathological morphology in rat hippocampus. The expression of Caspase-3 in hippocampus was detected by immunohistochemistry. Results Compared with the normal group, blood glucose level was significantly increased in the model group; the number of neurons in the hippocampus decreased in the model group; the apoptosis increased in the model group; the neuronal soma showed deformation and reduced dendritic spines and other pathological damages in the model group. After intervention of ZGJTJYP, hyperglycemia and depression-like status of the model rats were relieved; the number of hippocampal neurons increased significantly; neuronal morphology basically returned to normal. The expression of Caspase-3 in ZGJTJYP high-and medium-dose groups was significantly lower than that in model group. Conclusion ZGJTJYP can significantly improved blood glucose and learning and memory abilities in DD rats, which may be related to slowing hippocampal injury and apoptosis, and restoring neuronal quantity and morphology.关键词
左归降糖解郁方/糖尿病并发抑郁症/海马神经元/病理形态/大鼠Key words
Zuogui Jiangtang Jieyu Prescription/diabetes mellitus with depression/hippocampal neuron/pathological morphology/rats分类
医药卫生引用本文复制引用
柳卓,孟盼,杨蕙,赵洪庆,王宇红..左归降糖解郁方对糖尿病并发抑郁症大鼠海马神经元病理形态及凋亡的影响[J].中国中医药信息杂志,2018,25(9):44-48,5.基金项目
国家自然科学基金(81373578 （）
81403379 （）
81573965) （）
一方研究生科研创新项目(YF201714) （YF201714）
湖南省教育厅优秀青年资助项目(17B201) （17B201）
