肿瘤防治研究2018，Vol.45Issue(1)：5-8,4.DOI:10.3971/j.issn.1000-8578.2018.17.0388
石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制
Apoptosis Induction of Lycorine on Lung Cancer NCI-H460 Cells and Related Mechanism
摘要
Abstract
Objective To investigate the effect of lycorine on the proliferation inhibition and apoptosis induction in human lung cancer NCI-H460 cells, and to explore the anti-tumor mechanism of lycorine. Methods MTT assay was used to detect the proliferation of NCI-H460 cells. The apoptosis rate of NCI-H460 cells was detected by flow cytometry. The activity of Caspase 3 was detected by colorimetry. Results Lycorine could inhibit the proliferation of NCI-H460 cells, and the IC50 was (5.79±0.11) μmol/L. Lycorine had an induced effect on the apoptosis of NCI-H460 cells. Lycorine could enhance Caspase 3 activity. Conclusion Lycorine could effectively inhibit the proliferation and induce the apoptosis of NCI-H460 cells in vitro, and its mechanism may be related to the activation of Caspase 3 expression and the apoptosis induce of tumor cells.关键词
石蒜碱/肺癌/NCI-H460细胞/细胞凋亡/Caspase 3Key words
Lycorine/Lung cancer/NCI-H460 cells/Apoptosis/Caspase 3分类
医药卫生引用本文复制引用
李居伟,杨扬..石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制[J].肿瘤防治研究,2018,45(1):5-8,4.基金项目
广西自然科学基金(2014GXNSFAA118235) （2014GXNSFAA118235）
