肿瘤防治研究2018，Vol.45Issue(2)：61-66,6.DOI:10.3971/j.issn.1000-8578.2018.17.0809
敲除SLC39A5基因对4-NQO诱发C57BL/6小鼠食管癌模型建立的影响
Impact of SLC39A5 Knockout on Establishment of Esophageal Cancer Model Induced by 4-NQO in C57BL/6 Mice
摘要
Abstract
Objective To explore the influences of SLC39A5 knockout on the establishment of esophageal cancer model induced by 4-nitroquinoline 1-oxide (4-NQO) in C57BL/6 mice. Methods Ten wild-type mice were treated as negative control group and drank the 1,2-propylene glycol at 100 μg/ml; 140 wild-type mice and 80 knockout genotype mice were treated as experimental groups and drank the carcinogen 4-NQO stock solution dissolved in 1,2-propylene glycol at 100μg/ml, the experimental time was 28 weeks and all three groups' mice were sacrificed. Results The survival rates were 100％, 92.96％ and 91.25％ in negative group, wild-type experimental group and SLC39A5 knockout genotype experimental group, respectively; the rates of tumor formation were 0, 61.36％ and 28.77％, and there was a statistical difference between the two experimental groups(χ2=19.98, P＜0.001). Conclusion The esophageal cancer model in C57BL/6 mice and SLC39A5 knockout mice are established successfully and the facilitated role of SLC39A5 in the occurrence and development of esophageal cancer is verified.关键词
4-硝基喹啉-N-氧化物(4-NQO)/食管癌/SLC39A5基因敲除小鼠/C57BL/6野生型小鼠Key words
4-nitroquinoline 1-oxide(4-NQO)/Esophageal cancer/SLC39A5 knockout mice/C57BL/6 wild-type mice分类
医药卫生引用本文复制引用
王立群,靳晶,刘聪敏,高肇妤,梁迪,李道娟,郭甜甜,贺宇彤..敲除SLC39A5基因对4-NQO诱发C57BL/6小鼠食管癌模型建立的影响[J].肿瘤防治研究,2018,45(2):61-66,6.基金项目
国家自然科学基金(81272682) （81272682）
