肿瘤药学2019，Vol.9Issue(3)：396-400,5.DOI:10.3969/j.issn.2095-1264.2019.03.09
紫苏醇对结肠癌SW480细胞凋亡的影响及其机制研究★
Mechanism of Perillol-Induced Apoptosis in Colon Cancer SW480 Cells★
摘要
Abstract
Objective To study the effect of perillyl alcohol on apoptosis of colon cancer SW480 cells, and to reveal the specific mechanism of perillyl alcohol -induced apoptosis through Rspo3 and Notch signaling pathways. Methods Colon cancer SW480 cells were treated with different concentrations of apatinib (1, 5, 10 μg·mL-1) or perillyl alcohol (1, 5, 10 μg·mL-1). Intracellular Rspo3 and Notch-1 expressions were detected by Western-blot method. Detect the cell proliferation activity, cycle, invasion and metastasis ability, as well as the activity of apoptosis protein Cas-pase-3. Results Both perillyl alcohol and apatinib inhibited the expression of intracellular Rspo3 and Notch-1 and enhanced their inhibitory ability with increasing concentration (P<0.05). The inhibiting effect of perillyl alcohol on protein expression of Rspo3 and Notch-1 was much greater than that of apatinib at the same dose (P<0.05). Both perillyl alcohol and apatinib inhibited the proliferation and invasion of colon cancer SW480 cells, and their inhibitory ability was strengthened with the increase of concentrations (P<0.05). G0/G1 phase cells were significantly increased after treat-ment with perillyl alcohol and apatinib (P<0.05), but there was no significant change in G2/M phase cells (P>0.05). Perillyl alcohol and apafittin could not inhibit Caspase 3 activity at low dose (1 μg·mL-1) (P>0.05), but significantly increased the Caspase-3 activity in colon cancer SW480 cells at medium and high doses (5, 10 μg·mL-1) (P<0.05). Conclusion Perillyl alcohol can inhibit the proliferation of colon cancer SW480 cells and the expression of intracellular Notch-1 protein, and down-regulate the expression of Rspo3 gene and protein through feedback.关键词
Rspo3/结肠癌SW480细胞/Notch信号通路/Caspase 3/紫苏醇Key words
Rspo3/Colon cancer SW480 cells/Notch signaling pathway/Caspase 3/Perillyl alcohol分类
医药卫生引用本文复制引用
任建峰,张其胜,王慧..紫苏醇对结肠癌SW480细胞凋亡的影响及其机制研究★[J].肿瘤药学,2019,9(3):396-400,5.基金项目
国家自然科学基金(81472674). （81472674）
