兽类学报2018，Vol.38Issue(2)：183-191,9.DOI:10.16829/j.slxb.150110
基于细胞色素氧化酶亚基Ⅰ基因的中缅树鼩地理种群遗传分化
Genetic variation of different populations in tree shrews (Tupaia belangeri) based on mt DNA cytochrome oxidase subunit Ⅰ gene
摘要
Abstract
Tree shrews (Tupaia belangeri) are widely distributed in southeast Asia, mainly in Hainan Island and southwest regions of China. In the present study, PCR amplification of cytochrome oxidase subunit Ⅰ (COⅠ) gene sequence of1398 bp was used to analyze 10 geographic populations consisting of 112 tree shrews as the research objects. A total of 64 haplotypes were defined in COⅠ gene of the 112 samples, the haplotype diversity (Hd) averaged value was 0. 9730, and the average value of nucleotide diversity (Pi) was 0. 044 94; analysis of AMOVA showed that variation among populations accounted for 93. 09％ of the total variance, suggesting that mutations occurred mainly in populations. The overall genetic differentiation index (FST) fixed to 0. 930 91, indicating that the populations of tree shrews have obvious genetic differentiation. Combination with neutral inspection and mismatch distribution analysis showed that T. belangeri had not experienced population expansion phenomenon in history. A phylogenetic tree constructed of haplotypes and NETWORK diagram showed that 10 the geographic populations of tree shrews were clustered into 4 branches: Hainan population, Daxin population, Pianma population and other populations. All of the results showed that there was higher genetic diversity of different geographical populations of the China in T. belangeri. Different populations displayed obvious genetic differentiation and geographical barrier effects may be the main reason for the differentiation.关键词
中缅树鼩/COⅠ基因/遗传距离Key words
COⅠ gene/Mean genetic distance/Tupaia belangeri分类
生物科学引用本文复制引用
付家豪,王晓,朱万龙,高文荣,王政昆..基于细胞色素氧化酶亚基Ⅰ基因的中缅树鼩地理种群遗传分化[J].兽类学报,2018,38(2):183-191,9.基金项目
国家自然科学基金项目(31660121) （31660121）
云南省科技计划项目(2015GA008) （2015GA008）
云南省应用基础研究计划重点项目(2016FA045) （2016FA045）
