烟草科技2019，Vol.52Issue(7)：1-9,9.
不同浓度硒对烟草根系形态、生长素含量及相关基因表达的影响
Effects of selenium at different concentrations on root morphology, auxin content and auxin-related gene expression in tobacco
摘要
Abstract
In order to clarify the effects of selenium (Se) at different concentrations on root morphology, auxin contents and auxin-related gene expressions, an artificial climate incubator with light intensity of 300 μmol·m-2· s-1, 28℃/16 h in the day and 24℃/8 h in the evening was used to plant tobacco seedlings (homozygous DR5::GUS transgenic line, Yunyan 87). The root biomass, root length, lateral root number, MDA and auxin contents and the expression levels of NtYUCCA (a rate-limiting enzyme in auxin synthesis) and NtPIN (an auxin polar transport protein) gene families in transgenic tobacco were analyzed under Se treatments at concentrations of 0, 1, 5, 10 and 20 mg/L. The results showed that Se had double effects on tobacco growing. At low Se concentration (1 mg/L), the expression levels of NtYUCCA and NtPIN gene families, GUS activity, auxin content in root, biomass of tobacco plants, taproot length, lateral root number and antioxidant capacity increased, while MDA content decreased, indicating that the growing and development of tobacco were promoted. At high Se concentration (10 mg/L), auxin content and GUS activity reduced, suggesting that the growing and development of tobacco were inhibited.关键词
烟草/根系/硒/生长素/基因家族/NtYUCCA/NtPINsKey words
Tobacco/Root/Selenium/Auxin/Gene family/NtYUCCA/NtPINs分类
农业科技引用本文复制引用
罗勇,刘胜波,张涛,王立志,轩栋栋,徐涤寒,邵惠芳,贾宏昉..不同浓度硒对烟草根系形态、生长素含量及相关基因表达的影响[J].烟草科技,2019,52(7):1-9,9.基金项目
国家自然科学基金资助项目"高亲和磷转运蛋白OsPT8参与植物调控根系发育的作用机制"(31301837) （31301837）
河南省高等学校大学生实践创新训练计划项目"生长素在硒调控烟草根系发育过程中的作用机制研究"(201810466044). （201810466044）
