烟草科技2019，Vol.52Issue(7)：61-68,8.
气相色谱-串联质谱检测新鲜烟叶中的萜类成分
Determination of terpenoids in fresh tobacco leaves by GC-MS/MS
摘要
Abstract
In order to quantify tobacco terpenoids, a method for simultaneous determination of 21 terpenoids, including t,t-farnesol, α-phytol, cis-abienol, β-phytol, (1S,2E,4S,6R,7E,11E)-2,7,11-cembratriene-4,6-diol (α-CBD), (1S,2E,4R,6R,7E,11E)-2,7,11-cembratriene-4,6-diol (β-CBD), sclareol, labd-13-ene-8,15-diol, squalene, 2,3-oxosqualene, dihydrotachysterol, cholesterol, brassicasterol, ergosterol, campesterol, stigmasterol, lanosterol, β-sitosterol, β-amyrin, α-amyrin and chenodeoxycholic acid in tobacco was developed using gas chromatography triple quadruples mass spectrometry (GC-MS/MS). The characteristic fragment ion peaks of the 21 terpenoids were obtained under selective reaction monitoring (SRM) mode in positive channels. The results noted that under the optimized conditions, the determination coefficients (R2) of the 21 terpenoids were 0.999 0-0.999 8 in the linear ranges. The limits of detection (LOD) for analytes ranged from 12.7 to 308.7 ng·g-1, and the limits of quantification (LOQ) were 35.8-984.6 ng·g-1. The intra- and inter-day relative standard deviations (RSDs) were 0.19%-8.28% and 1.02%-9.35% respectively. Mature fresh tobacco leaves from Yunnan, Henan and Guizhou Provinces were analyzed using this method. The results showed that the total amount of α-CBD was Yunnan (98.83 μg·g-1) > Henan (57.04 μg·g-1) > Guizhou (38.61 μg·g-1), and there was significant difference among them. In particular, the terpenoid contents in leaves from Guizhou differed substantially from Yunnan and Henan, and that in leaves of cv. Nanjiang No.3 from Zunyi was significantly different from the other three varieties. This method uses only a simple pretreatment and is reproducible, can cover a wide range of terpenoids for batch analysis of tobacco leaves.关键词
烟草/萜类成分/气相色谱-三重四极杆质谱(GC-MS/MS)Key words
Tobacco/Terpenoid/Gas chromatography triple quadruples mass spectrometry分类
轻工纺织引用本文复制引用
郑庆霞,刘萍萍,陈千思,翟妞,徐国云,张慧,金立锋,陈霞,周会娜..气相色谱-串联质谱检测新鲜烟叶中的萜类成分[J].烟草科技,2019,52(7):61-68,8.基金项目
国家烟草专卖局重大专项项目"典型香型烟草品质形成的代谢特征研究"[110201401012(JY-12)]. （JY-12）
