烟草科技2019，Vol.52Issue(7)：69-74,6.
连续流动法快速测定基因编辑素材中淀粉含量
Rapid determination of starch in gene edited tobacco by continuous flow method
摘要
Abstract
In order to achieve high-throughput and accurate determination of starch in tobacco, a sample extraction vial was designed and used as a pre-treatment device to establish an ultra-fast method for determining starch. By using this extraction vial, the removal of interference components, the extraction and the filtration could be achieved without sample transfer. The results showed that: 1) The sample extraction vial integrated the functions of sample extraction, filtration and waste removal, which significantly improved preparation speed. 2) The linear relationship of starch was good (r=0.999 6). The mean recoveries ranged from 96.40% to 101.30% , and the intra-day and inter-day precision was less than 3.3%. 3) The contents of starch in eight gene edited tobacco samples were consistent with the data determined by the current tobacco industry standard method. The proposed method is simple, rapid, sensitive, reproducible and suitable for high-throughput starch detection in tobacco samples.关键词
淀粉/基因编辑素材/连续流动法Key words
Starch/Gene edited tobacco/Continuous flow method分类
轻工纺织引用本文复制引用
黄海涛,李晶,许永,王晋,刘欣,孔维松,杨叶昆,李雪梅,杨光宇,张承明..连续流动法快速测定基因编辑素材中淀粉含量[J].烟草科技,2019,52(7):69-74,6.基金项目
云南中烟工业有限责任公司科技项目"烟草苗期代谢缺陷研究及在基因育种筛选中的应用"(2018JC06). （2018JC06）
