南方医科大学学报2019，Vol.39Issue(6)：627-632,6.DOI:10.12122/j.issn.1673-4254.2019.06.01
人重组蛋白PDCD5抑制胶原诱导性关节炎大鼠来源的淋巴细胞增殖和炎性细胞因子分泌并促进活化的淋巴细胞凋亡
rhPDCD5 suppresses pro-inflammatory cytokine secretion and proliferation and induces apoptosis of activated lymphocytes from rats with collagen-induced arthritis
摘要
Abstract
Objective To investigate the effect of recombinant human PDCD5 (rhPDCD5) treatment in a rat model of bovine II collagen (CII)-induced arthritis (CIA) on inflammatory cytokine secretion,proliferation and apoptosis of activated lymphocytes and explore the mechanisms of rhPDCD5-induced immunosuppression on activated lymphocytes.Methods Female Wistar rats were randomly divided into normal control group,CIA+ ovalbumin (OVA) group,CIA+ rhTNFR:Fc group,and CIA+rhPDCD5 group.The rats in the latter 3 groups received intraperitoneal injections of OVA (14 mg/kg),rhTNFR:Fc (3.5 mg/kg) or rhPDCD5 (14 mg/kg) from day 2 to day 26 following CII injection.On day 28,the spleens of the rats were harvested for preparing single cell suspensions of splenocytes,which were activated by CII (20 μg/mL) or anti-CD3 (1 μg/mL)+anti-CD28 (2 μg/mL) for 48 h and 72 h.The production of interferon-y (IFN-y) and interleukin-17A (IL-17A) by the activated lymphocytes was determined by ELISA of the culture supematants.The proliferation and apoptosis of the activated lymphocytes were assessed using [3H]-thymidine incorporation assay and flow cytometry,respectively.Results Compared with those in CIA +OVA group,IFN-y and IL-17A secretions by the activated lymphocytes from rhPDCD5-treated CIA rats sgnfcantly decreased.RhPDCD5 treatment of the CIA rats obvously suppressed the prolferaton and promoted apoptoss of the lymphocytes actvated by CII or by ant-CD3 + ant-CD28.Concluson rhPDCD5 reduces pro-nflammatory cytokne secreton,nhibits the proliferation and promotes activation-induced cell death of activated CD4+ lymphocytes to produce immunosuppression in rat models of CIA.关键词
人重组蛋白PDCD5/淋巴细胞/活化诱导性凋亡/类风湿性关节炎Key words
recombinant human PDCD5/lymphocytes/activation-induced cell death/rheumatoid arthritis引用本文复制引用
WANG Wanlin,MAO Chun,XIAO Juan..人重组蛋白PDCD5抑制胶原诱导性关节炎大鼠来源的淋巴细胞增殖和炎性细胞因子分泌并促进活化的淋巴细胞凋亡[J].南方医科大学学报,2019,39(6):627-632,6.基金项目
国家自然科学基金(81601373) （81601373）
国家留学基金 （）
湖北省青年科技晨光计划 （）
襄阳市青年科技英才开发计划(No.[2018]46) （No.[2018]46）
湖北文理学院食品新型工业化学科群建设项目 （）
Supported by National Natural Science Foundation of China (81601373) （81601373）
National Fund for Studying Abroad,Hubei Youth Science and Technology Talent Development Plan,Xiangyang Youth Science and Technology Talent Development Plan (No.[2018]46) （No.[2018]46）
the Project for Discipline Groups Construction of New-type Food Industrialization of Hubei University of Arts and Science. （）
