化工学报2019，Vol.70Issue(7)：2675-2683,9.DOI:10.11949/0438-1157.20190076
蛹虫草菌深层液体发酵耦合大孔树脂吸附高效生产虫草素
Efficient production of cordycepin during submerged liquid fermentation by Cordyceps militaris coupled with macroporous resin adsorption
摘要
Abstract
Cordycepin, as a kind of nucleoside antibiotic, has various biological and pharmaceutical activities, such as anti-cancer and anti-tumor activities. Cordycepin is mainly obtained from liquid culture of Cordyceps militaris, whose low yield restricted its further application though. Considering the high concentration of cordycepin accumulation to produce strong feedback inhibition on its biosynthesis process, a strategy to remove some cordycepin and weaken the feedback inhibition to increase the production of cordycepin by fermentation separation coupling technique was proposed. Next, static adsorption test was taken and macroporous resin NKA-II was screened, followed by optimization of adsorption conditions such as resin usage (60 g·L-1), temperature (37℃), and desorption solvent (100% ethanol), giving 644.50 mg·L-1 cordycepin in fermentation under 30 g·L-1 initial glucose with repeated adsorption processing (twice), 32.07% higher than the control. To further improve the cordycepin yield, fed-batch fermentation coupled with resin adsorption separation was performed, giving a final yield of 787.50 mg·L-1, which was 35.78% higher than the control. It was found that the strategy of submerged liquid fermentation coupled with macroporous resin adsorption could efficiently weaken the feedback inhibition of cordycepin, and dramatically enhance the productivity of product, providing novel ideas for industrial cordycepin production.关键词
发酵/生物分离/生物过程/虫草素/蛹虫草菌Key words
fermentation/bioseparation/bioprocess/cordycepin/Cordyceps militaris分类
生物科学引用本文复制引用
GUAN Haiqing,LI Hongyu,LI Qian,LIU Bingnan,WANG Jihui,WANG Liang..蛹虫草菌深层液体发酵耦合大孔树脂吸附高效生产虫草素[J].化工学报,2019,70(7):2675-2683,9.基金项目
国家重点研发计划项目(2018YFC1406800) （2018YFC1406800）
国家自然科学基金项目(31500040) （31500040）
辽宁省科技厅自然科学基金计划重点项目(20170520102) （20170520102）
