临床神经病学杂志2019，Vol.32Issue(2)：126-130,5.
血红素加氧酶-1 在肢体缺血后处理对脑缺血再灌注损伤的保护作用
Protection of heme oxygenase-1 in limb ischemic postconditioning on cerebral ischemia reperfusion injury
摘要
Abstract
Objective To investigate the protection of heme oxygenase-1 ( HO-1 ) in limb ischemic postconditioning (LPostC) on cerebral ischemia reperfusion injury.Methods Eighty male SD rats were randomly divided into sham-operation group ( sham group ), ischemia reperfusion group ( I／R group ), limb ischemia postconditioning group ( LPostC group) and inhibitor of HO-1 group ( ZnPP group), 20 rats in each group.The ischemia and reperfusion injury model was established by line embolism with paraffin to block the middle cerebral artery.It includes three cycles of 5 min to occlusion and 5 min to loosen the bilateral femoral artery to make the LPostC model.After 24 h reperfusion, adopted 2,3,5-Triphenyltetrazolium chloride ( TTC) staining to measure cerebral infarction volume.TdT-mediated dUTP Nick-End Labeling (TUNEL) staining was used to detected neuronal apoptosis.Immunohistochemistry and Western blotting method were employed to detect the expression of HO -1. Malondialdehyde ( MDA ) content and Superoxide Dismutase ( SOD ) activity in brain tissue were measured by spectrophotometer.Results The expression of HO-1 and SOD activity were lower whereas MDA content was higher in I／R group than in sham group ( all P＜0.05).When compared with those in I／R group, the cerebral infarction volume diminished, and the number of nerve cell apoptosis significantly reduced , and the expression of HO-1 sharply increased, meanwhile SOD activity rose and MDA content decreased in LPostC group ( all P＜0.05).Cerebral infarction volume, nerve cell apoptosis and MDA content stronger , whereas the expression of HO-1 and SOD activity markedly lower in ZnPP group than in LPostC group ( all P＜0.05).Conclusion Limb ischemia postconditioning plays the role to protect cerebral ischemia reperfusion injury , and the mechanism may be related to the upregulation of HO-1.关键词
血红素加氧酶-1/肢体缺血后处理/缺血再灌注损伤Key words
heme oxygenase-1/ limb ischemic postconditioning / ischemia reperfusion injury分类
医药卫生引用本文复制引用
YANG Yun,LI Hao,ZHOU Qian..血红素加氧酶-1 在肢体缺血后处理对脑缺血再灌注损伤的保护作用[J].临床神经病学杂志,2019,32(2):126-130,5.基金项目
广西壮族自治区卫生厅科研课题( Z2014338) （ Z2014338）
桂林市科学研究与技术开发计划(20170109-11) （20170109-11）
