临床神经外科杂志Issue(2)：142-146,5.
与缺氧诱导因子-1 α调控相关的缝隙连接 43蛋白磷酸化参与脑缺血再灌注损伤的机制
Phosphorylation of Cx43 regulated by HIF-1αin cerebral ischemia-repufusion injury
摘要
Abstract
Objective To investigate the effect of inhibition of HIF-1α to alleviate the phosphorylation of Cx43 in the acute phase of cerebral ischemia-repufusion ( I／R ) injury. Methods One hundred Sprague-Dawley rats at 4 months age,weight at 300-500 g were randomly divided into 4 sections and 5 groups: sham operation, treament group ( I／R4,8 hour +2ME2 ), vehicle(I／R 8 hour+DMSO) and injury group (I／R 8 hour).Cerebral ischemia-repufusion injury model were established with clip bilateral ICA , and HIF-1αinhibitor 2ME2 was injected into peritoneal cavity.Half of rats were measured for cerebral water content by Hatashita wet-weight method.Another half rats were sacrificed for investigating the level of total-Cx43, phosphoylated Cx43, Bcl-2, Bax and Caspases-3 by western blot, TNF-α, IL-1βand IL-6 by Elisa kit.Result Unlike static total-Cx43, brain water content, phosphoylated Cx43, Bax, Caspases-3, and IL-1β, IL-6, TNF-αin each treatment group and sham group were significantly lower than in other groups. Bcl-2 by WB showed the opposite result to the above.Conclusion Inhibition of HIF-1αcan down-regulate the level of phosphoylation of Cx 43 and apoptosis factors.Further more, the level of IL-1, IL-6 and TNF-αwere reduced.Simultaneously,these alterations can protect the form and function of brain after cerebral ischemia-repufusion injury.关键词
缝隙连接43/缺氧诱导因子-1α/炎性因子/脑水肿/脑缺血再灌注损伤Key words
connexin43/ HIF-1α/ inflammatory factor/ cerebral edema/ cerebral ischemi repufusion injury分类
医药卫生引用本文复制引用
CHENWei,REN Da-bin,ZHENG Ping..与缺氧诱导因子-1 α调控相关的缝隙连接 43蛋白磷酸化参与脑缺血再灌注损伤的机制[J].临床神经外科杂志,2019,(2):142-146,5.基金项目
上海健康医学院种子基金( SFP-18-21-13-005) （ SFP-18-21-13-005）
上海健康医学院种子基金( SFP-18-21-13-007 ) （ SFP-18-21-13-007 ）
国家自然科学基金(81701231) （81701231）
上海市自然基金(16ZR1431500) （16ZR1431500）
