临床神经外科杂志Issue(2)：153-159,7.DOI:10.3969/j.issn.1672-7770.2019.02.012
下调磷酸甘油酸激酶 1 对胶质瘤 U373 细胞的放疗增敏作用及其机制的研究
Down-regulation of PGK1 ameliorate radiosensitivity in U373 human glioma cell and its mechanism
摘要
Abstract
Objective To explore the role of down-regulating phosphokinase 1 ( PGK1 ) in ameliorating the radiosensitivity of U 373 human glioma cell and the potential mechanism. Methods Radioresistant U373 cells ( RR-U373 ) were established.The short hairpinRNA (shRNA) for PGK1 were transfected to RR-U373 cells and normal U373 cells using Lipofectamine TM 2000.After transfection, they were treated with 5 Gy or 0 Gy irradiation.MTT assay, scratch assay and transwell assay were used to determine the relative survival , cell migration and cell invasion abilities, respectively.Western Blot was uesd to evaluate PGK 1, CIN and CFL1 expressions.Results Down-regulating PGK1 using shRNA decreased cell viability ,migration and invasion abilities both before and after radiation.After down-regulation of PGK1, the expression of CIN and CFL1 protein decreased.Conclusion These findings suggest that down-regulating PGK1 could enhance radiosensitivity in U 373 human glioma cell and the efficacy may be associated with the regulation effect of PGK1 on CIN and CFL1.关键词
磷酸甘油酸激酶1/ CIN/丝切蛋白1/胶质瘤U373细胞/放疗敏感性Key words
phosphokinase 1/ CIN/ CFL1/ U373 human glioma cell/ radiosensitivity分类
医药卫生引用本文复制引用
LIU Yu-chi,ZHAO Meng-jie,SUN Wen-bo..下调磷酸甘油酸激酶 1 对胶质瘤 U373 细胞的放疗增敏作用及其机制的研究[J].临床神经外科杂志,2019,(2):153-159,7.基金项目
国家自然科学基金面上项目(81172390 ) （81172390 ）
南京市医学科技发展重大项目(ZDX16011) （ZDX16011）
