临床神经外科杂志2019，Vol.16Issue(3)：235-240,244,7.DOI:10.3969/j.issn.1672-7770.2019.03.011
AHR／SOCS2通路调控氧糖剥夺再灌注星形胶质细胞炎性反应的研究
AHR／SOCS2 pathway regulates astrocyte inflammatory response induced by cerebral ischemia reperfusion injury
摘要
Abstract
Objective To investigate the role of AHR／SOCS2 pathway on astrocyte inflammatory response after oxygen deprivation.Methods Astrocytes ( AS) were isolated and cultured in vitro to establish astrocyte oxygen glucose deprivation (OGD／R) model.AS were divided into the following groups as ctrl group (normal culture ), OGD／R group, OGD／R+negative control group, OGD／R+AHR overexpression group , OGD／R+AHR overexpression +SOCS2 knockdown group.Western blot and RT-PCR were used to detect the expression of AHR , SOCS2 and inflammatory factors in each group.It was used to confirm whether SOCS2 could block the expression of AS inflammatory factors TNF-α, IL-6 and iNOS after OGD／R which was regulated by AHR.Results The expression levels of TNF-α, IL-6 and iNOS were increased after overexpression of AHR and SOCS2.Knocking down SOCS2 could block the up-regulation of TNF-α, IL-6 and iNOS which was caused by AHR.Conclusion The AHR／SOCS2 pathway is involved in the regulation of AS inflammation after OGD／R.关键词
星形胶质细胞/氧糖剥夺再灌注/ AHR/ SOCS2/炎性因子Key words
astrocyte/OGD／R/AHR/SOCS2/inflammatory factors分类
医药卫生引用本文复制引用
YE Han-bin,HUANG Wei-yi,REN Bing-yan,et al...AHR／SOCS2通路调控氧糖剥夺再灌注星形胶质细胞炎性反应的研究[J].临床神经外科杂志,2019,16(3):235-240,244,7.基金项目
国家自然科学基金(81701216) （81701216）
江苏省自然科学青年基金(BK20160197) （BK20160197）
江苏省自然科学基金( BK20161140) （ BK20161140）
中国无锡青年医学人才( QNRC086) （ QNRC086）
