肿瘤预防与治疗2019，Vol.32Issue(4)：299-304,6.DOI:10. 3969/j. issn. 1674-0904. 2019. 04. 002
Matrigel三维培养模型对乳腺癌细胞整合素β1的定位及胞外基质粘附的影响
Effects of 3D Matrigel Model on the Localization of Integrin β1 of Breast Cancer Cells and the Adhesion Ability of Breast Cancer Cells to the Extra-cellular Matrix
摘要
Abstract
Objective: To investigate the the effects of matrigel, a 3D culture model, on the morphology, localization of integrin β1 and adhesion ability of breast cancer cells to laminin, fibronectin and collagen IV; study the different interactions of breast cancer cells and extracellular matrix (ECm) in 2D and 3D culture models; and reveal the effect of tumor microenvi-ronment on tumor cells. Methods: mDA-mB-435S and mDA-mB-231, breast cancer cells, were cultured with 2D and 3D matrigel models. The viability of cells was tested by Calcein Am. The localization of integrin β1 was studied with immunos-taining. And the adhesion ability of breast cancer cells to ECm were measured by adhesion assay. Results: The localization of integrin β1 exhibited significant difference between 2D and 3D cultures. mediated by integrin β1 in 3D models, the adhe-sion ability of breast cancer cells to laminin increased significantly and that to fibronectin and collagen IV decreased signifi- cantly. Conclusion: 3D culture model can mimic the 3D microenvironment in vivo and can be used as a new study model to reveal the interaction of tumor cells and ECm and the effects of tumor microenvironment on tumor progression.关键词
乳腺癌/整合素β1/胞外基质蛋白/matrigel/三维培养模型Key words
Breast cancer/Integrin β1/Extracellular matrix proteins/matrigel/3D culture model分类
医药卫生引用本文复制引用
Gu Cuirong,Liu Huifen,Wang Hao,Huang Lingxiao,Huang Jianming,mi Kun..Matrigel三维培养模型对乳腺癌细胞整合素β1的定位及胞外基质粘附的影响[J].肿瘤预防与治疗,2019,32(4):299-304,6.基金项目
国家自然科学基金(编号: 81872402) This study was supported by the National Natural Science Foundation of China (NO. 81872402). （编号: 81872402）
