肿瘤预防与治疗2019，Vol.32Issue(5)：395-401,7.DOI:10. 3969/j. issn. 1674-0904. 2019. 05. 003
微小 RNA-181a-5P、320a 及其靶基因 TGF-β1 在食管鳞癌中的表达*
ExPression of miR-181a-5P, miR-320a and TGF-β1 in EsoPhageaI Squamous CeII Carcinoma
摘要
Abstract
To investigate the exPression of miR-181a-5P, miR-320a and TGF-β1 in esoPhagea1 squamous ce11 carcino-ma and study their ro1es in the deve1oPment of esoPhagea1 squamous ce11 carcinoma. Methods: Three samP1es of esoPhagea1 squamous ce11 carcinoma and adjacent norma1 tissue were co11ected. The miRNA array was used to detect the exPression of miRNA. The re1iabi1i-ty of the miRNAs chiP resu1ts was verified using rea1-time quantitative Po1ymerase chain reaction (qRT-PCR). Differentia11y exPressed miRNA-regu1ated target genes were ana1yzed by software and database. miR-181a-5P, miR-320a and TGF-β1, extracted from 22 cases of esoPhagea1 squamous ce11 carcinoma and 22 cases of norma1 tissue, were verified using qRT-PCR. ResuIts: The miRNA chiP resu1ts showed that 22 miRNAs differentia11y exPressed in esoPhagea1 squamous ce11 carcinoma comPared with those in norma1 tissue ( P<0. 05). Six miRNAs significant1y increased, whi1e 16 significant1y decreased. It was confirmed that resu1ts of qRT-PCR were consistent with those of the miRNA array. qRT-PCR confirmed that the exPression of miR-320a decreased and that of miR-181a-5P increased in esoPhagea1 squamous ce11 carcinoma comPared with those in norma1 tissue ( P < 0. 05 ). The exPression of TGF-β1 in esoPhagea1 squamous ce11 carcinoma was s1ight1y higher than that in norma1 tis-sue, but the difference was not statistica11y significant. ConcIu- sion: miR-181a-5P and miR-320a may be engaged in carcinogenesis of esoPhagea1 squamous ce11 carcinoma.关键词
miR-181a-5P/miR-320a/食管鳞癌/miRNA芯片/qRT-PCR/TGF-β1Key words
miR-181a-5P/miR-320a/EsoPhagea1 squamouce11 carcinoma/MicroRNA array/Qrt-PCR/TGF-β1引用本文复制引用
Li Yanhua,Li Yushan,Zhang Renjing,Zhou Xuemei,He Xinrong..微小 RNA-181a-5P、320a 及其靶基因 TGF-β1 在食管鳞癌中的表达*[J].肿瘤预防与治疗,2019,32(5):395-401,7.基金项目
四川省教育厅课题(编号:14ZA0200) This study was suPPorted by grants from Education DePartment of Sichuan Province (NO. 14ZA0200). （编号:14ZA0200）
