山西农业大学学报（自然科学版）2019，Vol.39Issue(1)：3792-3797,6.DOI:10.13842／j.cnki.issn1671‐8151.201810018
黄壤伯克氏溶磷细菌的筛选鉴定及溶磷特性
Screening ,identification and characteristic analysis of phosphorus solubilizing bacteria Burkholderia sp.in yellow soil
摘要
Abstract
[Objective]Present study was conducted in order to discover new phosphorus solubilizing bacteria strains and to im‐prove the bioavailability of soil phosphorus in yellow soil area .[Methods] Phosphorus solubilizing bacteria (PSB) strains were isolated from yellow soil of Guizhou by PVK plate method .[Results] After a few of isolation and purification steps ,fourteen bacteria strains with phosphate solubilizing capacity (PSC ) greater than 395 mg·L－1 were obtained.Among of them ,W4 showed PSC at 564.07 mg·L－1 ,which was significantly higher than other strains (P＜0.05).Therefore ,W4 was selected for further studies on characteristics of PSB .Based on 16sRNA gene sequence results ,W4 was identified as Burkholderia sp. When the glucose concentration of culture medium was lower than 10 g·L －1 ,the PSC of W4 was significantly affected by the content of the glucose (P＜0.05) ,while when glucose concentration was greater than 10 g·L－1 ,no significant effect of glu‐cose content on PSC of W4 was observed .When glucose concentration in culture medium was greater than 15 g·L －1 ,the glu‐conic acid produced by bacteria activity was increased which inhibited the bacteria growth rate .With the increase of ammonium sulfate concentration in the culture medium ,the PSC of W4 was gradually decreased ,while the growth rate of bacteria was in‐creased significantly (P＜0.05).Among the different carbon and nitrogen sources ,glucose and potassium nitrate produced the highest PSC of W4 at 604.10 mg·L －1 .The addition of phosphorus inhibited PSC of W4.[Conclusion] The results of present study provided evidence for better understanding the characteristics of phosphorus‐soluble bacteria in yellow soil and provided theoretical basis for further bacteria application .关键词
黄壤/伯克氏菌/筛选鉴定/溶磷特性Key words
Yellow soil/Burkholderia sp/Screening and identification/Phosphate‐solubilizing characteristics引用本文复制引用
Qiao Zhiwei,Teng Feilong,Shao Xiaogui..黄壤伯克氏溶磷细菌的筛选鉴定及溶磷特性[J].山西农业大学学报（自然科学版）,2019,39(1):3792-3797,6.基金项目
贵州省科技计划项目(黔科合基础20181401) （黔科合基础20181401）
贵州省科技厅、安顺市人民政府、安顺学院三方联合基金项目(黔科合 L H 字2016‐7280) （黔科合 L H 字2016‐7280）
贵州省普通高等学校科技拔尖人才项目(黔教合 KY 字2017091) （黔教合 KY 字2017091）
安顺学院博士科研启动项目(asubsjj201601) （asubsjj201601）
