山西农业大学学报（自然科学版）2019，Vol.39Issue(1)：3798-3803,6.DOI:10.13842／j.cnki.issn1671‐8151.201809052
锦灯笼提取物对 6 种植物病原真菌的抑制作用研究
Antifungal effects of extracts of Physalis alkekengi L.var.f ranchetii on six phytopathogenic fungus species
摘要
Abstract
[Objective]The aim of present research was to identify active plant components with strong antifungal activity and provide solid evidence for novel botanical antifungal agent development .[M ethods ] Chinese herbal plant , Physalis alkekengi ,was extracted with petroleum ether ,ethyl acetate ,acetone ,or water ,and the antifungal activities of different crude extracts were determined with the mycelium grow th rate method ,and the protective and therapeutic effects of ethyl acetate extract w hich produced the highest mycelium grow th inhibition activity were investigated against A lternaria solani by in vivo method .[Results] The results showed that the ethyl acetate extract exhibited greater antifungal activity than other extracts ,especially against V alsa m ali and A lternaria solani ,and the inhibition rates were up to 73. 53% and 83. 41% at 96 h ,respectively .M eanw hile ,the EC50 values of ethyl acetate extract against A lternaria solani was 0. 79 g· L －1 ,and the EC50 values of ethyl acetate extract against V alsa m ali and Fusarium spp .were 1. 30 g·L －1 and 1. 44 g· L －1 ,respectively .The protective and therapeutic effects of ethyl acetate extract against A lternaria solani in tomato fruit were measured ,and the protective function of ethyl acetate extract was higher than 50%,and the therapeutic effect was reached up to 65% at the concentration of 10 g·L －1 .[Conclusion] The results indicated a bunch of secondary metabolites existing in Physalis alkekengi w hich exhibited excellent antifungal effects against many phytopathogenic fungi and pos‐sessed the potential to be developed as botanic antifungal agents .关键词
锦灯笼/提取物/植物病原真菌/抗菌活性/植物源抗菌剂Key words
Physalis alkekengi/Crude extracts/Phytopathogenic fungi/Antifungal activity/Botanic antifungal agent引用本文复制引用
Yang Chun,Li Ting,Jiang Lingyun,Zhi Xiaoyan,Wang Yi,Cao Hui..锦灯笼提取物对 6 种植物病原真菌的抑制作用研究[J].山西农业大学学报（自然科学版）,2019,39(1):3798-3803,6.基金项目
国家自然科学基金(31700300 ,21702129) （31700300 ,21702129）
山西农业大学科技创新基金(2016YJ09 ,2016YJ10 ) （2016YJ09 ,2016YJ10 ）
山西功能农业共性关键技术研究与示范(201703D211001‐01‐04) （201703D211001‐01‐04）
