山西农业大学学报（自然科学版）2019，Vol.39Issue(1)：3818-3825,8.DOI:10.13842／j.cnki.issn1671‐8151.201808009
蝎蝽次目线粒体基因组 22 个 tRN A 基因的比较研究(半翅目:异翅亚目)
Comparative study of 22 mitochondrial tRNA genes in Nepomorpha (Hemiptera :Heteroptera)
摘要
Abstract
[Objective] Present study was conducted to comparatively analyze the sequence character ,genetic distance , percentage of identical nucleotides ,and the secondary structure of 22 tRNA genes from 11 families of Nepomorpha and to construct phylogeny based on those determined gene informaiton .[M ethods ] All tRNA genes of 12 representative species of Nepomorpha were obtained from GenBank .Sequence character and genetic distance were computed in M EGA 6.0 .Percentage of identical nucleotides was calculated in BioEdit 7.1 .T he secondary structure of tRNA genes were constructed in RNA structure 5.8 ,and phylogenetic trees were constructed in RAxM L 8.2.8 and M rbayes 3.2.5 . [Results] T he results showed that the average nucleotide composition of (A+ T )% was 76.41% .T he content of both A and T were higher than G and C ,and A was higher than T in most species with G higher than C in all species .T he genetic distance between inter‐families was 0.13～0.26 .T he tRNA‐L2 ,tRNA‐T ,and tRNA‐M genes with higher nu‐cleotide conservation were located in the major strand ,and the tRNA‐V and tRNA‐F genes with low nucleotide conser‐vation were located in the minority strand .T he amino acid acceptor stem in the secondary structure showed a higher conservation ,w hile the anticodon loops were relatively conservative and the other loops had different degrees of base variation .T he phylogenetic results showed that Pleoidea ,Notonectoidea ,and Corixoidea were all monophyletic ,and Pleoidea and Notonectoidea were sister groups .T he monophyly of other superfamilies were not well received ,w hich might be associated with less effective phylogenetic signals that tRNA gene sequences provided .[Conclusion ] T his study provided positive impact on knowledge gap of Nepomorpha tRNA and revealed the importance of tRNA gene and its secondary structure in comparative mitochondrial genomic study .关键词
蝎蝽次目/线粒体基因组/tRNA/比较研究/系统发育Key words
Nepomorpha/M itochondrial DNA/tRNA/Comparative study/Phylogeny引用本文复制引用
Zhang Danli,Zhang Miaomiao,Tian Jing,Li Yuerui,Zhang Hufang..蝎蝽次目线粒体基因组 22 个 tRN A 基因的比较研究(半翅目:异翅亚目)[J].山西农业大学学报（自然科学版）,2019,39(1):3818-3825,8.基金项目
山西省来晋博士奖励科研启动基金(I170258) （I170258）
太原师范学院博士科研启动基金(I170174) （I170174）
