山西农业大学学报（自然科学版）2019，Vol.39Issue(1)：3893-3897,5.DOI:10.13842／j.cnki.issn1671‐8151.201807019
羊骨胶原肽对去卵巢大鼠的骨质改善作用
Sheep bone collagen peptide improve bone quality of ovariectomized rats
摘要
Abstract
[Objective] T he positive effect of sheep bone collagen peptide (CP ) on bone quality of ovariectomized rats was investigated to provide solid evidence for adding‐value utilization of animal bone resources and non‐medicine pre‐vention of osteoporosis .[M ethods] Bilateral ovariectomized rats were divided into six groups ,including ovariectomized (OVX) ,estrogen (E2 ) ,CP at high level (CP‐H ) ,CP at medium level (CP‐M ) ,CP at low level (CP‐L groups) ,and SHAM group which were sham operated .Eight rats were involved in each group .In addition to basal diet for all groups ,CP groups were also intragastrically administered with CP at different dosages ,w hile SHAM and OVX group were treated with equal‐volume distilled water ,and rats in E2 group were injected with 17‐βestrodial .After nine weeks treatment ,femoral bones of rats in each group were sampled to determine the bone density , bone mineral , and hydroxyproline content ,and blood samples were collected to determine serum PINP and β‐CT x levels .[Results] Re‐sults showed that the bone density ,bone minerals (Ca ,M g ,Zn ,M n) ,and hydroxyproline content of OVX group ((1.767 ± 0.031 ) g·cm －3 ,(9.73 ± 0.14 ) mg·g －1 ,(0.34 ± 0.01 ) mg·g －1 ,(30.48 ± 1.48 ) μg·g －1 ,(1.70 ± 0.24 ) μg·g －1 ,and (1.922 ± 0.12 ) μg·mg －1 ,respectively ) were significantly lower than those of SHAM group ((2.283 ± 0.47) g·cm －3 ,(12.59 ± 2.78 ) mg·g －1 ,(0.49 ± 0.10) mg·g －1 ,(33.78 ± 0.43) μg·g －1 ,(1.94 ± 0.29) μg·g －1 ,and (2.258 ± 0.22 ) μg·mg －1 , respectively ).T he serum PINP and β‐CT x levels of OVX at (3528.37 ± 154.74 ) and (529.67 ± 23.63 ) pg·mL －1 were significantly higher than those in SHAM group at (2258.55 ± 140.87 ) and (370.98 ± 61.89) pg·mL －1 ( P＜0.05) ,which indicated that osteoporosis model was successfully established .Bone density , bone mineral ,and hydroxyproline content in both CP groups and E2 group were significantly greater than those in the OVX group ,while serum PINP and β‐CT x levels were significantly lower than OVX group (P＜0.05).[Conclusion] Sheep bone collagen peptide can inhibit the loss of bone mineral and bone organic matter in ovariectomized rats and can be an important role in bone quality improvement .关键词
羊骨胶原肽/骨密度/骨矿物质/PINP ,β‐CT x/羟脯氨酸Key words
Sheep bone collagen peptide/Bone density/Bone minerals/PINP/β‐CT x/Hydroxyproline引用本文复制引用
Yuan Kai,Li Ailing,Han Keguang,Hao Zailin,Cheng Zhilin,Chen Jing,Jin Shuxiu,Ma Lin‐feng,Huo Nairui..羊骨胶原肽对去卵巢大鼠的骨质改善作用[J].山西农业大学学报（自然科学版）,2019,39(1):3893-3897,5.基金项目
国家自然科学基金(31201347) （31201347）
山西省回国留学人员项目(2014‐042) （2014‐042）
