山西农业大学学报（自然科学版）2019，Vol.39Issue(5)：54-61,8.DOI:10.13842／j.cnki.issn1671-8151.201902001
基于HPLC法测定不同产地黄芪总黄酮和异黄酮的含量
Determination of total flavonoids and isoflavones content in Radix Astragali from different production areas by HPLC
摘要
Abstract
[Objective ] Present study was conducted to explore the differences of the content of flavonoids and isoflavones in A stragalus samples from different habitats .[M ethods] T he contents of total flavonoids and four isofla-vones (isoflavone glycosides ,ononin ,calycosin and fermononetin ) of A stragalus from 16 different areas were meas-ured and comparedby HPLC ,and analyzed using principal component analysis method .[Results ] T he differences of the total content of flavonoids and four isoflavones were detected in the samples from different habitats ,while they were not significantly different between species A .membranaceus and A .mongholicus .T he content of the isoflavone glucoside of 5 species out of 16 matched the requirement of pharmacopoeia with no less than 0 .02% content standard . T he results of the main component comprehensive score analysis showed the highest scoreat Dai county ,followed by Guangling county ,and Zeqingling village of Hunyuan county of Shanxi province ,and the radix A stragali grow n in these areas were belonged to species A .mongholicus .Samples of A stragalus species from 16 different regions were classified into three categories with SIMCA-P Principal Component Analysis method .[Conclusion] T he differences be-tween the components of A .membranaceus and A .mongholicus were not statistically significant ,and they could be used as the basic species of Radix A stragali .However ,the total content of flavonoids and isoflavones of A stragalus in dif-ferent inhabits were significantly different ( P＜ 0.05 ).T he production areas where the content of active ingredients meet the standard requirement should be considered when planting Radix A stragali .关键词
黄芪/总黄酮/异黄酮类/HPLCKey words
A stragalus/Total flavonoids/Isoflavones/HPLC分类
医药卫生引用本文复制引用
Liu Yaling,Geng Yaping,Huang Xiaosu,Zhao Linru,Zhang Pengfei..基于HPLC法测定不同产地黄芪总黄酮和异黄酮的含量[J].山西农业大学学报（自然科学版）,2019,39(5):54-61,8.基金项目
国家自然科学基金项目(31400285) （31400285）
