山西农业大学学报（自然科学版）2019，Vol.39Issue(5)：82-89,8.DOI:10.13842／j.cnki.issn1671-8151.201902019
地熊蜂非视觉相关beta-arrestin基因的克隆与表达谱分析
Cloning and expression spectrum analysis of non-visual beta-arrestin gene in the Bombus terrestris
摘要
Abstract
[Objectives] T he aims of present study were to clone and identify the non-visual beta-arrestin gene of Bombus-terrestris ,to analyze its expression variation at different developmental stages and in specific tissues of queen bees ,and to lay a foundation for the functional research of target gene .[M ethods]PCR amplification and gene cloning were used to obtain the full-length cDNA sequence of the non-visual related beta-arrestin gene.T he sequences of amino acids encoded by the gene were compared and analyzed using bioinformatics techniques .Real-time PCR was used to analyze the gene expression in the brain ,ovary ,midgut ,and fatbody of queens at different developmental stages ,including one-day be-fore pharate stage ,one day after callow stage ,mated for 5-days ,hibernation for 3 months ,hibernation for 5 months , egg-laying stage ,and senescent stage .[Results]T he CDS sequence of beta-arrestin gene was 1248 bp long ,and the size of the encoded protein was 415 aa.T he 27～182 amino acid residues in the amino acid sequence of the protein were deter-mined as the conserved domain of A rrestin-N superfamily ,and the 201～354 amino acid residues were the conserved do-main of A rrestin-C superfamily .T he similarity analysis indicated that the amino acid sequence of the beta-arrestin gene of bumblebee was consistent with that of the transcriptome data collected in NCBI up to 97% .Real-time PCR results showed that beta-arrestin gene was expressed in all developmental stages and tested tissues ,but the expression levels were significantly different .[Conclusion] In this study ,the CDS sequence of bumblebee beta-arrestin gene was cloned ;the amino acid sequence encoded by beta-arrestin gene cDNA was determined ;the sequence characteristics and expres-sion profile of this gene were analyzed ,and it was speculated that it was related to the physiological metabolic process and hormone secretion activity during the queen′s overwinter period .关键词
地熊蜂/beta-arrestin基因/基因克隆/表达分析Key words
Bombus terrestris/Beta-arrestin/Gene cloning/Expression analysis分类
农业科技引用本文复制引用
Tang Peng,Zhao Mohan,Yang Huipeng,Su Songkun,Wu Jie..地熊蜂非视觉相关beta-arrestin基因的克隆与表达谱分析[J].山西农业大学学报（自然科学版）,2019,39(5):82-89,8.基金项目
国家蜂产业技术体系专项资金(CARS-44) （CARS-44）
中国农业科学院科技创新工程(CA ASAS TIP-2019-IAR) （CA ASAS TIP-2019-IAR）
国家现代农业产业技术体系(蜜蜂)项目(No .CARS-44-KXJ4) （蜜蜂）
